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PMID:3753653
| Citation |
Blau, N and Niederwieser, A (1986) The application of 8-aminoguanosine triphosphate, a new inhibitor of GTP cyclohydrolase I, to the purification of the enzyme from human liver. Biochim. Biophys. Acta 880:26-31 |
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| Abstract |
GTP cyclohydrolase I from human liver and Escherichia coli is competitively inhibited by 8-aminoguanosine triphosphate with a dissociation constant (Ki) of 0.25 mumol/l. 8-Aminoguanosine triphosphate, prepared from GTP and hydroxylamine-O-sulfonic acid, was coupled to Sepharose 4B and used as an affinity adsorbent for a 309-fold purification of GTP cyclohydrolase I from human liver. GTP cyclohydrolase I from human liver is a relatively heat-stable enzyme with a half-life of 2 min at 80 degrees C, an isoelectric point (pI) of about 5.6, and a Km for GTP of 31 mumol/l. Addition of KCl (0.3 mol/l) increased the Km to 153 mumol/l. No cofactors were required for activity. L-erythro-5,6,7,8-Tetrahydrobiopterin, L-erythro-7,8-dihydrobiopterin, L-sepiapterin and 8-aminoguanosine triphosphate were strong inhibitors. |
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| Keywords |
Aminohydrolases/isolation & purification; Chromatography, Affinity/methods; GTP Cyclohydrolase/antagonists & inhibitors; GTP Cyclohydrolase/isolation & purification; Guanosine Triphosphate/analogs & derivatives; Guanosine Triphosphate/chemical synthesis; Guanosine Triphosphate/metabolism; Guanosine Triphosphate/pharmacology; Humans; Liver/enzymology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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