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PMID:3753435
| Citation |
Beeley, JG, Eason, R and Snow, DH (1986) Isolation and characterization of latherin, a surface-active protein from horse sweat. Biochem. J. 235:645-50 |
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| Abstract |
A protein, latherin, with unusual surface activity was isolated from horse sweat by gel filtration and ion-exchange chromatography. The protein has a Stokes radius, determined by gel filtration, of 2.47 nm, and in the ultracentrifuge sediments as a single species with S20,W 2.05 S, indicating an Mr of 24,400. On SDS/polyacrylamide-gel electrophoresis the molecule behaves as a single peptide chain of apparent Mr 20,000. Latherin contains a high proportion of hydrophobic amino acids (37.2%), and the leucine content (24.5%) is exceptionally high. The unusual composition of the protein may account for apparent anomalies in the Mr of latherin determined by empirical methods. Evidence indicating that latherin is responsible for much of the surface activity of horse sweat was obtained by a simple assay for surface tension and by contact-angle measurements. Latherin adsorbs very readily at hydrophobic surfaces, rendering them wettable. A possible role for latherin in thermoregulation is proposed. |
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| Keywords |
Amino Acids/analysis; Animals; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Horses/metabolism; Molecular Weight; Proteins/isolation & purification; Surface Tension; Surface-Active Agents/isolation & purification; Sweat/analysis |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0050828: regulation of liquid surface tension |
ECO:0000314: |
P |
Figure 3. |
complete | ||||
| GO:0001659: temperature homeostasis |
ECO:0000314: |
P |
Table 4 shows that Latherin adsorbs readily to hydrophobic surfaces, rendering them wettable by water at concentrations one or more orders of magnitude lower than of other proteins. This property of the protein may be of functional importance in temperature regulation, as it allows sweat to spread over the animal rather than beading. |
complete | ||||
|
involved_in |
GO:0050828: regulation of liquid surface tension |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0001659: temperature homeostasis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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