PMID:3624233

Citation	Goliger, JA and Roberts, JW (1987) Bacteriophage 82 gene Q and Q protein. Sequence, overproduction, and activity as a transcription antiterminator in vitro. J. Biol. Chem. 262:11721-5
Abstract	Phage 82 gene Q encodes a phage-specific positive regulator of late gene expression, thought, by analogy to the corresponding gene of phage lambda, to be a transcription antiterminator. We have cloned and sequenced the phage 82 gene Q and have overproduced and purified the 82 Q protein. We also have identified and sequenced DNA containing the phage 82 late gene promoter and terminator. We show that purified 82 Q protein is active and specific for DNA containing the 82 late gene promoter in a well defined in vitro transcription reaction: RNA polymerase initiating at the phage 82 late gene promoter and modified by 82 Q protein reads through a downstream transcriptional terminator. We used T1 RNase mapping to confirm that the putative readthrough RNA made in the presence of 82 Q protein is in fact an elongation product of the shorter RNA.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Cloning, Molecular; Coliphages/genetics; Gene Expression Regulation; Genes, Viral; Peptide Chain Termination, Translational; Promoter Regions, Genetic; Terminator Regions, Genetic; Transcription, Genetic; Viral Proteins/genetics; Viral Proteins/isolation & purification; Viral Proteins/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BP82:REGQ		GO:0031564: transcription antitermination	ECO:0000314:		P		Figure 5 shows that as [82Q] increases, the amount of terminator readthrough increases.	complete
BP82:REGQ		GO:0001173: DNA-dependent transcriptional start site selection	ECO:0000314:		P		Figure 6 shows RNA initiating at the phage 82 late gene promoter and terminating 80 base pairs downstream.	complete CACAO 6471

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