PMID:3519853

Citation	Atlan, D, Lazzaroni, JC and Portalier, R (1986) Effects of pho regulatory mutations and phoA gene amplification on alkaline phosphatase synthesis and release by lky mutants of Escherichia coli K12. J. Gen. Microbiol. 132:171-81
Abstract	lky mutants of Escherichia coli K12 spontaneously released alkaline phosphatase (APase) into the extracellular medium to give up to 300 units ml-1. APase is a phosphate repressible periplasmic enzyme encoded by the gene phoA. With a view to establishing a method of easy purification, we have analysed APase synthesis and release patterns of isogenic lky strains containing either a constitutive pho regulatory mutation, or a hybrid plasmid carrying the structural gene phoA+ and pho regulatory genes, or a transducing phi 80 phoA+ phage. In the presence of the phoS2333 mutation, F- lky strains lysogenized with phi 80 phoBin phoA+ phage and grown in high phosphate medium were able to release eight times more APase activity (2300 units ml-1) than haploid strain 2336 (phoS+ lky) grown in low phosphate medium. Neither protein synthesis, the cell export machinery nor leakage mechanisms were limiting for APase release. Sufficient APase was released into the medium to facilitate its purification.
Links	PubMed
Keywords	Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Extracellular Space/analysis; Gene Amplification; Genes, Regulator; Lysogeny; Mutation; Plasmids
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLX:Q47485	Contributes to	GO:0004035: alkaline phosphatase activity	ECO:0000270:		F		in figure 2,3, and 4 show expression of gene through mutant interactions	complete

References

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