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PMID:3318829
| Citation |
Schoedon, G, Curtis, HC and Niederwieser, A (1987) Localization of GTP cyclohydrolase I in human peripheral blood smears using a specific monoclonal antibody and an immune-alkaline phosphatase labeling technique. Biochem. Biophys. Res. Commun. 148:1232-6 |
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| Abstract |
GTP cyclohydrolase I, the enzyme catalyzing the first step in the cofactor biosynthesis for the aromatic amino acid hydroxylases, has been localized in situ. By the use of a monoclonal antibody specific to human GTP cyclohydrolase I, the enzyme has been visualized immuno-enzymatically by alkaline phosphatase monoclonal anti-alkaline phosphatase labeling. In routine blood smears lymphocytes, monocytes/macrophages, and granulocytes show strong intraplasmatic staining. Premature erythrocytes show clear staining of the reticulated cytoplasmatic structure, while mature erythrocytes are completely negative. Neither is there any staining for GTP cyclohydrolase I in the blast cells of a case of T-cell acute lymphoblastic leukemia. These results closely confirm the prior finding that mature erythrocytes as well as most malignant mononuclear cells lack GTP cyclohydrolase I activity, and they indicate that in these cells the enzyme protein may be absent. |
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| Keywords |
Alkaline Phosphatase/diagnostic use; Aminohydrolases/blood; Antibodies, Monoclonal/diagnostic use; Biopterin/analogs & derivatives; Biopterin/biosynthesis; Blood Cells/enzymology; GTP Cyclohydrolase/blood; Humans; Immunoenzyme Techniques |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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