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PMID:3298686
| Citation |
García, JL, García, E, Arrarás, A, García, P, Ronda, C and López, R (1987) Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin. J. Virol. 61:2573-80 |
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| Abstract |
Cp-1, a small virulent bacteriophage infecting Streptococcus pneumoniae, encodes its own lytic enzyme (CPL). A fragment of Cp-1 DNA containing the gene cpl coding for CPL was cloned and expressed in high amounts in Escherichia coli. CPL was purified to electrophoretic homogeneity by using affinity chromatography on choline-Sepharose (T. Briese and R. Hakenbeck, Eur. J. Biochem. 146:417-427, 1985), and the enzyme showing a Mr of 39,000 was characterized as a muramidase. This muramidase required for in vivo and in vitro activity the presence of choline in the teichoic acids of the pneumococcal cell walls. Free choline or lipoteichoic acid noncompetitively inhibited the activity of CPL. |
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| Keywords |
Bacteriophages/genetics; Cloning, Molecular; Enzymes/genetics; Enzymes/isolation & purification; Escherichia coli; Genes, Viral; Muramidase/genetics; Protein Biosynthesis; Streptococcus pneumoniae; Structure-Activity Relationship; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0003796: lysozyme activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044659: cytolysis by virus of host cell |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0003796: lysozyme activity |
ECO:0000314: |
F |
Figure 7. Shows presence of CPL leads to lysis of host bacterial cells. Control strains are shown in the same figure. |
complete | ||||
| GO:0044659: cytolysis by virus of host cell |
ECO:0000314: |
P |
Figure 7. Shows presence of CPL leads to lysis of host bacterial cells. Control strains are shown in the same figure. |
complete | ||||
Notes
See also
References
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