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PMID:31048338
| Citation |
Sun, Y, Wollman, AJM, Huang, F, Leake, MC and Liu, LN (2019) Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment. Plant Cell 31:1648-1664 |
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| Abstract |
The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO fixation and potentials in bioengineering applications, the formation of carboxysomes and their structural composition, stoichiometry, and adaptation to cope with environmental changes remain unclear. Here we use live-cell single-molecule fluorescence microscopy, coupled with confocal and electron microscopy, to decipher the absolute protein stoichiometry and organizational variability of single β-carboxysomes in the model cyanobacterium PCC7942. We determine the physiological abundance of individual building blocks within the icosahedral carboxysome. We further find that the protein stoichiometry, diameter, localization, and mobility patterns of carboxysomes in cells depend sensitively on the microenvironmental levels of CO and light intensity during cell growth, revealing cellular strategies of dynamic regulation. These findings, also applicable to other bacterial microcompartments and macromolecular self-assembling systems, advance our knowledge of the principles that mediate carboxysome formation and structural modulation. It will empower rational design and construction of entire functional metabolic factories in heterologous organisms, for example crop plants, to boost photosynthesis and agricultural productivity. |
| Links |
PubMed PMC6635877 Online version:10.1105/tpc.18.00787 |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031470: carboxysome |
ECO:0007107: yellow fluorescent protein fusion protein localization evidence used in manual assertion |
C |
Fig. 2 and Supp. Fig. 6 show YFP-tagged CcmM is localized in the carboxysomes. UniProtKB: Q03513 (CCMM_SYNE7) Organism: Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) UniProt submitted name: Carbon dioxide concentrating mechanism protein CcmM |
complete | ||||
| GO:0031470: carboxysome |
ECO:0007107: yellow fluorescent protein fusion protein localization evidence used in manual assertion |
C |
Fig. 2 and Supp. Fig. 6 show YFP-tagged CcmK4 localized in the carboxysomes. UniProtKB: Q03511 (CCMK_SYNE7) Organism: Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) UniProt submitted name: Carbon dioxide-concentrating mechanism protein CcmK |
complete | ||||
| GO:0031470: carboxysome |
ECO:0007107: yellow fluorescent protein fusion protein localization evidence used in manual assertion |
C |
Supp. Fig. 6 show YFP-tagged CcmL localized in the carboxysomes. UniProtKB: Q03512 (CCML_SYNE7) Organism: Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) UniProt submitted name:Carbon dioxide concentrating mechanism protein CcmL |
complete | ||||
Notes
See also
References
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