GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:30545912
| Citation |
Duval, M, Dar, D, Carvalho, F, Rocha, EPC, Sorek, R and Cossart, P (2018) HflXr, a homolog of a ribosome-splitting factor, mediates antibiotic resistance. Proc. Natl. Acad. Sci. U.S.A. 115:13359-13364 |
|---|---|
| Abstract |
To overcome the action of antibiotics, bacteria have evolved a variety of different strategies, such as drug modification, target mutation, and efflux pumps. Recently, we performed a genome-wide analysis of gene expression after growth in the presence of antibiotics, identifying genes that are up-regulated upon antibiotic treatment. One of them, , is a homolog of , which encodes a heat shock protein that rescues stalled ribosomes by separating their two subunits. To our knowledge, ribosome splitting has never been described as an antibiotic resistance mechanism. We thus investigated the role of in antibiotic resistance. First, we demonstrated that is an antibiotic resistance gene that confers protection against lincomycin and erythromycin, and that we renamed ( resistance). We show that expression is regulated by a transcription attenuation mechanism relying on the presence of alternative RNA structures and a small ORF encoding a 14 amino acid peptide containing the RLR motif, characteristic of macrolide resistance genes. We also provide evidence that HflXr is involved in ribosome recycling in presence of antibiotics. Interestingly, possesses another copy of , , that is not involved in antibiotic resistance. Phylogenetic analysis shows several events of duplication in prokaryotes and widespread presence of in Firmicutes. Overall, this study reveals the as the founding member of a family of antibiotic resistance genes. The resistance conferred by this gene is probably of importance in the environment and within microbial communities. |
| Links |
PubMed PMC6310831 Online version:10.1073/pnas.1810555115 |
| Keywords |
|
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0032790: ribosome disassembly |
ECO:0000315: |
P |
Fig. 3 shows that HflX (also known in the paper as lmo0762) in Listeria monocytogenes is responsible for ribosome recycling (aka ribosome disassembly). |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
