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PMID:30404215
Citation |
Yuan, Y, Peng, Q, Yang, S, Zhang, S, Fu, Y, Wu, Y and Gao, M (2018) Isolation of A Novel Phage Representing A New Phage Lineage and Characterization of Its Endolysin. Viruses 10 |
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Abstract |
Phages, the parasites of bacteria, are considered as a new kind of antimicrobial agent due to their ability to lyse pathogenic bacteria. Due to the increase of available phage isolates, the newly isolated phage showed increasing genomic similarities with previously isolated phages. In this study, the novel phage vB_BthS_BMBphi, infecting the strain BMB171, is isolated and characterized together with its endolysin. This phage is the first tadpole-like phage infecting the Bacillus strains. Genomic analysis shows that the phage genome is dissimilar to all those of previously characterized phages, only exhibiting low similarities with partial regions of the prophages. Phylogenetic analysis revealed that the phage was distant from the other Bacillus phages in terms of evolution. The novel genome sequence, the distant evolutionary relationship, and the special virion morphology together suggest that the phage vB_BthS_BMBphi could be classified as a new phage lineage. The genome of the phage is found to contain a restriction modification system, which might endow the phage with immunity to the restriction modification system of the host bacterium. The function of the endolysin PlyBMB encoded by the phage vB_BthS_BMBphi was analyzed, and the endolysin could lyse all the tested group strains, suggesting that the endolysin might be used in controlling pathogenic group strains. The findings of this study enrich the understanding of phage diversity and provide a resource for controlling the group pathogenic bacteria. |
Links |
PubMed PMC6266608 Online version:10.3390/v10110611 |
Keywords |
Bacillus Phages/classification; Bacillus Phages/isolation & purification; Bacillus Phages/physiology; Bacillus Phages/ultrastructure; Bacillus thuringiensis/virology; Bacteriolysis; Computational Biology/methods; Endopeptidases/genetics; Genome, Viral; Phylogeny; Viral Plaque Assay; Whole Genome Sequencing |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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GO:0044659: cytolysis by virus of host cell |
ECO:0001565: cell-based assay evidence |
P |
Fig. 7A measures effect of concentration on the the lytic activity of gp41 Fig. 7B measures effect of pH on the lytic activity of gp41 Submitted name in UniProt: N-acetylmuramoyl-L-alanine amidase UniProtKB - A0A494RGB7 (A0A494RGB7_9CAUD) Organism - Bacillus phage vB_BthS_BMBphi |
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Notes
See also
References
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