PMID:30190308

Citation	Marino, ND, Zhang, JY, Borges, AL, Sousa, AA, Leon, LM, Rauch, BJ, Walton, RT, Berry, JD, Joung, JK, Kleinstiver, BP and Bondy-Denomy, J' (2018) Discovery of widespread Type I and Type V CRISPR-Cas inhibitors. Science '
Abstract	Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here we report the discovery of 12 genes, including inhibitors of type V-A and I-C CRISPR systems. Notably, AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a when assayed in human cells. The genes reported here provide useful biotechnological tools and mark the discovery of loci in many bacteria and phages.
Links	PubMed Online version:10.1126/science.aau5174
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9GAMM:A0A0U2BNN7		GO:0099048: CRISPR-cas system	IDA: Inferred from Direct Assay:	P	Fig. 3 demonstrates that the acrVA1 protein effectively prevents various CRISPR Cas12a nucleases, including MbCas12a, Mb3Cas12a, AsCas12a, LbCas12a and FnCas12a from modifying DNA. NTR #16423, waiting for response. These proteins are negatively regulating this process.	complete CACAO 13383
9GAMM:A0A0U2BNP2		GO:0099048: CRISPR-cas system	IDA: Inferred from Direct Assay:	P	Fig. 2C and E demonstrates that acrVA2, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type V-A CRISPR, but not against Type I -C. NTR #16423, awaiting response.	complete CACAO 13428
9GAMM:A0A0U2B983		GO:0099048: CRISPR-cas system	ECO:0000314:		P		Fig. 2C and E demonstrates that acrVA2.1, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type V-A CRISPR, but not against Type I -C. NTR #16423, awaiting response.	complete CACAO 13430
9GAMM:A0A0U2BNH1		GO:0099048: CRISPR-cas system	IDA: Inferred from Direct Assay:	P	Fig. 2C and E demonstrates that acrIC1, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type I-C CRISPR, but not against Type V-A. NTR #16423, awaiting response.	complete CACAO 13432
9PSED:A0A1I0UR49		GO:0099048: CRISPR-cas system	ECO:0000314:		P		Fig. 2B demonstrates that acrIE5, an uncharacterized protein from Pseudomonas otitidis, inhibits the effect of DNA modification by type I-E CRISPR, but not against Type I -F. NTR #16423, awaiting response.	complete CACAO 13493
PSEAI:A6N5E2		GO:0099048: CRISPR-cas system	ECO:0000314:		P		Fig. 2B demonstrates that acrIF12, an uncharacterized protein from Pseudomonas aeruginosa, inhibits the effect of DNA modification by type I-F CRISPR, but not against Type I-E. NTR #16423, awaiting response.	complete CACAO 13495

References

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