PMID:28800709

Citation	Chen, H, Kandel, PP, Cruz, LF, Cobine, PA and De La Fuente, L (2017) The Major Outer Membrane Protein MopB Is Required for Twitching Movement and Affects Biofilm Formation and Virulence in Two Xylella fastidiosa strains. Mol. Plant Microbe Interact. 30:896-905
Abstract	MopB is a major outer membrane protein (OMP) in Xylella fastidiosa, a bacterial plant pathogen that causes losses on many economically important crops. Based on in silico analysis, the uncharacterized MopB protein of X. fastidiosa contains a β-barrel structure with an OmpA-like domain and a predicted calcium-binding motif. Here, MopB function was studied by mutational analysis taking advantage of the natural competence of X. fastidiosa. Mutants of mopB were constructed in two different X. fastidiosa strains, the type strain Temecula and the more virulent WM1-1. Deletion of the mopB gene impaired cell-to-cell aggregation, surface attachment, and biofilm formation in both strains. Interestingly, mopB deletion completely abolished twitching motility. Electron microscopy of the bacterial cell surface revealed that mopB deletion eliminated type IV and type I pili formation, potentially caused by destabilization of the outer membrane. Both mopB mutants showed reduced virulence using tobacco (Nicotiana tabacum) as a host under greenhouse conditions. These results suggest that MopB has pleiotropic functions in biofilm formation and twitching motility and is important for virulence of X. fastidiosa.
Links	PubMed Online version:10.1094/MPMI-07-17-0161-R
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
XYLFT:Q87AV4		GO:0043107: type IV pilus-dependent motility	ECO:0000315:		P		MopB is required for bacterial type IV pili twitching, as observed in figure 3. Organism: X. Fastidiosa	complete CACAO 12958
XYLFT:Q87AV4		GO:0044407: single-species biofilm formation in or on host organism	ECO:0000315:		P		Figure 1 shows that biofilm formation of X. fastidiosa strains under different conditions. It can be seen that mopB is involved, and important for the formation of biofilm. Organism: X. fastidiosa	complete CACAO 12959
XYLFT:Q87AV4		GO:0043683: type IV pilus biogenesis	ECO:0000315:		P		Figure 4 shows that mopB for pili biogenesis is required, as no pili were observed on mopB mutants. Organisms: X. fastidiosa	complete CACAO 12960
XYLFT:Q87AV4		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 5, virulence (or pathogenesis) of X. fastidiosa on tobacco plants where mopB affected severity of virulence on the leaves of the plant.	complete CACAO 12961
XYLFT:Q87AV4		GO:0009297: pilus assembly	ECO:0000315:		P		Figure 4 shows that mopB for pili biogenesis is required, as no pili were observed on mopB mutants. This annotation is being done ahead of the NTR for type I pilus biogenesis. As figure 4 reveals both type I and type IV (type IV I have alread annotated). Future GO term will be along the lines of "type I pilus biogenesis". Organisms: X. fastidiosa	complete CACAO 12973

References

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