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PMID:28408916
| Citation |
Chersicola, M, Kladnik, A, Tušek Žnidarič, M, Mrak, T, Gruden, K and Dermastia, M (2017) 1-Aminocyclopropane-1-Carboxylate Oxidase Induction in Tomato Flower Pedicel Phloem and Abscission Related Processes Are Differentially Sensitive to Ethylene. Front Plant Sci 8:464 |
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| Abstract |
Ethylene has impact on several physiological plant processes, including abscission, during which plants shed both their vegetative and reproductive organs. Cell separation and programmed cell death are involved in abscission, and these have also been correlated with ethylene action. However, the detailed spatiotemporal pattern of the molecular events during abscission remains unknown. We examined the expression of two tomato ACO genes, LeACO1, and LeACO4 that encode the last enzyme in ethylene biosynthesis, 1-aminocyclopropane-1-carboxylate oxidase (ACO), together with the expression of other abscission-associated genes involved in cell separation and programmed cell death, during a period of 0-12 h after abscission induction in the tomato flower pedicel abscission zone and nearby tissues. In addition, we determined their localization in specific cell layers of the flower pedicel abscission zone and nearby tissues obtained by laser microdissection before and 8 h after abscission induction. The expression of both ACO genes was localized to the vascular tissues in the pedicel. While LeACO4 was more uniformly expressed in all examined cell layers, the main expression site of LeACO1 was in cell layers just outside the abscission zone in its proximal and distal part. We showed that after abscission induction, ACO1 protein was synthesized in phloem companion cells, in which it was localized mainly in the cytoplasm. Samples were additionally treated with 1-methylcyclopropene (1-MCP), a competitive inhibitor of ethylene actions, and analyzed 8 h after abscission induction. Cell-layer-specific changes in gene expression were observed together with the specific localization and ethylene sensitivity of the hallmarks of cell separation and programmed cell death. While treatment with 1-MCP prevented separation of cells through inhibition of the expression of polygalacturonases, which are the key enzymes involved in degradation of the middle lamella, this had less impact on the occurrence of different kinds of membrane vesicles and abscission-related programmed cell death. In the flower pedicel abscission zone, the physical progressions of cell separation and programmed cell death are perpendicular to each other and start in the vascular tissues. |
| Links |
PubMed PMC5374216 Online version:10.3389/fpls.2017.00464 |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Colocalizes with |
GO:0005737: cytoplasm |
ECO:0000314: |
C |
Eight hours after abscission induction, many ACO1 gold-labeled particles (ACO1) specifically appeared in the cytoplasm of the phloem companion cells in the vascular tissues distal to the abscission zone in Solanum lycopersicum (Tomato) (Lycopersicon esculentum) shown in Fig. 2B by author. |
complete | |||
|
Colocalizes with |
GO:0009506: plasmodesma |
ECO:0000314: |
C |
Occasionally signals of the ACO1 gold-labeled particles (ACO1) were also seen in the plasmodesmata eight hours after the abscission in Solanum lycopersicum (Tomato) (Lycopersicon esculentum) Fig. 2B Protein: P05116 (ACCO1_SOLLC) |
complete | |||
|
Colocalizes with |
GO:0009505: plant-type cell wall |
ECO:0000314: |
C |
Occasionally signals of the ACO1 gold-labeled particles (ACO1) was also seen in the cell walls eight hours after the abscission shown in Fig. 2B. Protein: P05116 (ACCO1_SOLLC) Organism: Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
complete | |||
| GO:0005694: chromosome |
ECO:0000314: |
C |
Protein: E3 ubiquitin-protein ligase pellino homolog 1 Organism: Mus musculus (Mouse) Mitotic chromosomes of non-Tg and Peli1-Tg splenocytes were spread and visualized by Giemsa staining in Fig. 1B |
complete | ||||
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Colocalizes with |
GO:0000776: kinetochore |
ECO:0000314: |
C |
Protein: E3 ubiquitin-protein ligase pellino homolog 1 Organism: Mus musculus (Mouse) The graph represents the percentages of Peli1 co-localized with BubR1 at kinetochores in asynchronized or synchronized cells. By author in Fig. 2A and 2B |
complete | |||
Notes
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