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PMID:28286321
| Citation |
Udvari, EB, Völgyi, K, Gulyássy, P, Dimén, D, Kis, V, Barna, J, Szabó, ÉR, Lubec, G, Juhász, G, Kékesi, KA and Dobolyi, Á (2017) Synaptic proteome changes in the hypothalamus of mother rats. J Proteomics 159:54-66 |
|---|---|
| Abstract |
To establish synaptic proteome changes associated with motherhood, we isolated synaptosome fractions from the hypothalamus of mother rats and non-maternal control females at the 11th postpartum day. Proteomic analysis by two-dimensional differential gel electrophoresis combined with mass spectrometric protein identification established 26 significant proteins, 7 increasing and 19 decreasing protein levels in the dams. The altered proteins are mainly involved in energy homeostasis, protein folding, and metabolic processes suggesting the involvement of these cellular processes in maternal adaptations. The decrease in a significantly altered protein, complement component 1q subcomponent-binding protein (C1qbp) was validated with Western blotting. Furthermore, immunohistochemistry showed its presence in hypothalamic fibers and terminals in agreement with its presence in synaptosomes. We also found the expression of C1qbp in different hypothalamic nuclei including the preoptic area and the paraventricular hypothalamic nucleus at the protein and at the mRNA level using immunohistochemistry and in situ hybridization histochemistry, respectively. Bioinformatical network analysis revealed that cytokines, growth factors, and protein kinases are common regulators, which indicates a complex regulation of the proteome change in mothers. The results suggest that maternal responsiveness is associated with synaptic proteins level changes in the hypothalamus, and that growth factors and cytokines may govern these alterations. |
| Links |
PubMed Online version:10.1016/j.jprot.2017.03.006 |
| Keywords |
Animals; Female; Hypothalamus/metabolism; Nerve Tissue Proteins/biosynthesis; Postpartum Period/metabolism; Postpartum Period/physiology; Proteome/biosynthesis; Rats; Rats, Wistar |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005739: mitochondrion |
ECO:0006003: electron microscopy evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005739: mitochondrion |
ECO:0006003: electron microscopy evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0098793: presynapse |
ECO:0006003: electron microscopy evidence used in manual assertion |
C |
part_of:(UBERON:0001898) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0098978: glutamatergic synapse |
ECO:0006003: electron microscopy evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0098982: GABA-ergic synapse |
ECO:0006003: electron microscopy evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
Notes
See also
References
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