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PMID:28258146
| Citation |
Yao, GW, Duarte, I, Le, TT, Carmody, L, LiPuma, JJ, Young, R and Gonzalez, CF (2017) A Broad-Host-Range Tailocin from Burkholderia cenocepacia. Appl. Environ. Microbiol. 83 |
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| Abstract |
Thecomplex (Bcc) consists of 20 closely related Gram-negative bacterial species that are significant pathogens for persons with cystic fibrosis (CF). Some Bcc strains are highly transmissible and resistant to multiple antibiotics, making infection difficult to treat. A tailocin (phage tail-like bacteriocin), designated BceTMilo, with a broad host range against members of the Bcc, was identified instrain BC0425. Sixty-eight percent of Bcc representing 10 species and 90% of non-Bccstrains tested were sensitive to BceTMilo. BceTMilo also showed killing activity againstPAO1 and derivatives. Liquid chromatography-mass spectrometry analysis of the major BceTMilo proteins was used to identify a 23-kb tailocin locus in a draft BC0425 genome. The BceTMilo locus was syntenic and highly similar to a 24.6-kb region on chromosome 1 ofJ2315 (BCAL0081 to BCAL0107). A close relationship and synteny were observed between BceTMilo andphage KL3 and, by extension, with paradigm temperate myophage P2. Deletion mutants in the gene cluster encoding enzymes for biosynthesis of lipopolysaccharide (LPS) in the indicator strainK56-2 conferred resistance to BceTMilo. Analysis of the defined mutants in LPS biosynthetic genes indicated that an α-d-glucose residue in the core oligosaccharide is the receptor for BceTMilo.BceTMilo, presented in this study, is a broad-host-range tailocin active againstspp. As such, BceTMilo and related or modified tailocins have potential as bactericidal therapeutic agents against plant- and human-pathogenic. |
| Links |
PubMed PMC5411513 Online version:10.1128/AEM.03414-16 |
| Keywords |
Anti-Bacterial Agents/chemistry; Anti-Bacterial Agents/metabolism; Anti-Bacterial Agents/pharmacology; Bacteriocins/chemistry; Bacteriocins/metabolism; Bacteriocins/pharmacology; Burkholderia cenocepacia/chemistry; Burkholderia cenocepacia/genetics; Burkholderia cenocepacia/metabolism; Burkholderia cepacia complex/drug effects; Burkholderia cepacia complex/growth & development; Genome, Bacterial; Genome, Viral; Host Specificity; Humans; Mass Spectrometry; Molecular Structure; Pseudomonas aeruginosa/drug effects; Pseudomonas aeruginosa/growth & development |
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