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PMID:27258092

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Citation

Kot, W, Kilstrup, M, Vogensen, FK and Hammer, K (2016) Clear Plaque Mutants of Lactococcal Phage TP901-1. PLoS ONE 11:e0155233

Abstract

We report a method for obtaining turbid plaques of the lactococcal bacteriophage TP901-1 and its derivative TP901-BC1034. We have further used the method to isolate clear plaque mutants of this phage. Analysis of 8 such mutants that were unable to lysogenize the host included whole genome resequencing. Four of the mutants had different mutations in structural genes with no relation to the genetic switch. However all 8 mutants had a mutation in the cI repressor gene region. Three of these were located in the promoter and Shine-Dalgarno sequences and five in the N-terminal part of the encoded CI protein involved in the DNA binding. The conclusion is that cI is the only gene involved in clear plaque formation i.e. the CI protein is the determining factor for the lysogenic pathway and its maintenance in the lactococcal phage TP901-1.

Links

PubMed PMC4892519 Online version:10.1371/journal.pone.0155233

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

9CAUD:O48503

GO:0098689: latency-replication decision

ECO:0000315:

P

Figure 1 and Table 1 show mutant on different part of CI binding site< right before the site, on the site, or right after the site, would cause clear plaques or not.

complete
CACAO 12882

Notes

See also

References

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