GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:27249668
| Citation |
Raman, R, Damle, I, Rote, R, Banerjee, S, Dingare, C and Sonawane, M (2016) aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis. Nat Commun 7:11643 |
|---|---|
| Abstract |
Epithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis and have functions in mucous retention, membrane storage and abrasion resistance. It is largely unknown how the formation of these laterally long actin projections is regulated. Here, we show that antagonistic interactions between aPKC and Lgl-regulators of apical and basolateral domain identity, respectively,-control the length of microridges in the zebrafish periderm, the outermost layer of the epidermis. aPKC regulates the levels of Lgl and the active form of non-muscle myosinII at the apical cortex to prevent actin polymerization-dependent precocious fusion and elongation of microridges. Our data unravels the functional significance of exclusion of Lgl from the apical domain in epithelial cells. |
| Links |
PubMed PMC4895443 Online version:10.1038/ncomms11643 |
| Keywords |
Actin Cytoskeleton/genetics; Actin Cytoskeleton/metabolism; Actin Cytoskeleton/ultrastructure; Actins/genetics; Actins/metabolism; Animals; Animals, Genetically Modified; Cell Polarity; Embryo, Nonmammalian; Embryonic Development/genetics; Epidermal Cells; Epidermis/growth & development; Epidermis/metabolism; Epithelial Cells/metabolism; Epithelial Cells/ultrastructure; Gene Expression Regulation, Developmental; Glycoproteins/antagonists & inhibitors; Glycoproteins/genetics; Glycoproteins/metabolism; Morpholinos/genetics; Morpholinos/metabolism; Myosin Type II/genetics; Myosin Type II/metabolism; Protein Kinase C/genetics; Protein Kinase C/metabolism; Zebrafish/genetics; Zebrafish/growth & development; Zebrafish/metabolism; Zebrafish Proteins/antagonists & inhibitors; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0030031: cell projection assembly |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100419-12 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0030031: cell projection assembly |
ECO:0000316: genetic interaction evidence used in manual assertion |
ZFIN:ZDB-GENE-011105-1 |
P |
Seeded From UniProt |
complete | ||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
