PMID:26904379

Citation	LeBlanc, L, Nezami, S, Yost, D, Tsourkas, P and Amy, PS Isolation and characterization of a novel phage lysin active against Paenibacillus larvae, a honeybee pathogen. Bacteriophage 5:e1080787
Abstract	Paenibacillus larvae is the causative agent of American foulbrood (AFB) disease which affects early larval stages during honeybee development. Due to its virulence, transmissibility, capacity to develop antibiotic resistance, and the inherent resilience of its endospores, Paenibacillus larvae is extremely difficult to eradicate from infected hives which often must be burned. AFB contributes to the worldwide decline of honeybee populations, which are crucial for pollination and the food supply. We have isolated a novel bacteriophage lysin, PlyPalA, from the genome of a novel Paenibacillus larvae bacteriophage originally extracted from an environmental sample. PlyPalA has an N-terminal N-acetylmuramoyl-L-alanine amidase catalytic domain and possesses lytic activity against infectious strains of Paenibacillus larvae without harming commensal bacteria known to compose the honeybee larval microbiota. A single dose of PlyPalA rescued 75% of larvae infected with endospores, showing that it represents a powerful tool for future treatment of AFB. This represents the first time that lysins have been tested for therapeutic use in invertebrates.
Links	PubMed PMC4743491 Online version:10.1080/21597081.2015.1080787
Keywords
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Significance

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:A0A0K2CYE7		GO:0009253: peptidoglycan catabolic process	ECO:0000314:		P		Figures 3, 4 and 5 show PlyPalA has lytic activity on P. larvae cells. Table 1 showed that PlyPalA had lytic activity against different P. larvae genotypes and the lytic activity varied from genotype-to-genotype. ERIC genotype I members were the most susceptible to PlyPalA lytic activity	complete CACAO 12885

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