GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:26716769
| Citation |
Tu, S, Guo, SJ, Chen, CS, Liu, CX, Jiang, HW, Ge, F, Deng, JY, Zhou, YM, Czajkowsky, DM, Li, Y, Qi, BR, Ahn, YH, Cole, PA, Zhu, H and Tao, SC (2015) YcgC represents a new protein deacetylase family in prokaryotes. Elife 4 |
|---|---|
| Abstract |
Reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. However, only a few lysine deacetylases (KDACs) have been identified in prokaryotes, perhaps in part due to their limited sequence homology. Herein, we developed a 'clip-chip' strategy to enable unbiased, activity-based discovery of novel KDACs in the Escherichia coli proteome. In-depth biochemical characterization confirmed that YcgC is a serine hydrolase involving Ser200 as the catalytic nucleophile for lysine deacetylation and does not use NAD(+) or Zn(2+) like other established KDACs. Further, in vivo characterization demonstrated that YcgC regulates transcription by catalyzing deacetylation of Lys52 and Lys62 of a transcriptional repressor RutR. Importantly, YcgC targets a distinct set of substrates from the only known E. coli KDAC CobB. Analysis of YcgC's bacterial homologs confirmed that they also exhibit KDAC activity. YcgC thus represents a novel family of prokaryotic KDACs. |
| Links |
PubMed PMC4709262 Online version:10.7554/eLife.05322 |
| Keywords |
Amidohydrolases/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Lysine/metabolism; Protein Processing, Post-Translational; Substrate Specificity; Transcription Factors/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0034983: peptidyl-lysine deacetylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0033558: protein deacetylase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0034983: peptidyl-lysine deacetylation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
