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PMID:2612428

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Citation

Van Gossum, A, Loriers, M, Serruys, E and Cremer, M (1989) Methods of disinfecting endoscopic material: results of an international survey. Endoscopy 21:247-50

Abstract

A questionnaire on the methods of cleaning and disinfecting endoscopic equipment was mailed to 120 centers in Western Europe. Seventy-four questionnaires (61%) were returned. The centers were classified into three groups according to the number of endoscopic procedures performed per year. This survey has shown that precautions are more rigid in specialized centers than in units doing fewer than 1,500 endoscopic investigations a year. Complete disinfection is done by almost all the centers after ERCP in infected patients or in the case of HBsAg and HIV-positive patients, but only by 70% of centers after completion of endoscopies performed in patients with gastrointestinal hemorrhage and unknown immunological status. After endoscopic examination performed in presumably non-infected subjects, complete disinfection is not done in any center after gastroscopy, in 13% after colonoscopy and in 30% after ERCP. Continued education of endoscopic personnel and greater availability of adequate endoscopic equipment is mandatory for minimizing the risk of endoscopy-related infections.

Links

PubMed Online version:10.1055/s-2007-1012962

Keywords

Disinfection/methods; Endoscopes; Humans; Questionnaires; Sterilization/methods

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

AGRT5:A9CIM3

GO:0008654: phospholipid biosynthetic process

ECO:0000314:

P

Figure 2B shows that Phosphatidylcholine synthase uses choline to directly synthesize PC. Figure 2 (A&B) shows that Pcs is active when expressed and is likely responsible for PC synthesis without the requirement of additional proteins.

complete

AGRT5:A9CIM3

GO:0072657: protein localization to membrane

ECO:0000314:

P

Figure 6 shows that A. tumefaciens has significant levels of PC in both the inner and outer membranes, indicating Pcs activity in these locations.

complete

AGRT5:A9CIM3

GO:0048870: cell motility

ECO:0000315:

P

Figure 7 shows that A. tumefaciens is highly motile, and that the motility of the pmtA/pcs double mutant was greatly decreased. This figure also showed that when the pcs mutant was complimented with pcs expressed from a low copy number vector, motility was partially restored.

complete

AGRT5:A9CIM3

GO:0042710: biofilm formation

ECO:0000315:

P

Figure 8 shows that a PC-deficient double mutant (pcs/pmtA) formed thicker and more dense communities in comparison to wild type. It shows that there were numerous large, towering structures present.

complete


See also

References

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