GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:25825077
| Citation |
Yang, J, Ng, TB, Lin, J and Ye, X' (2015) A novel laccase from basidiomycete Cerrena sp.: cloning, heterologous expression and characterization. Int. J. Biol. Macromol. ' |
|---|---|
| Abstract |
A novel laccase gene Lac1 and its cDNA were cloned from a white-rot fungus Cerrena sp. and characterized. The 1,554-bp cDNA of Lac1 encoded a mature protein with 497 amino acids, preceded by a signal peptide of 20 amino acids. An unconventional intron splice site and incomplete splicing variants of Lac1 were observed. Lac1 was heterologously expressed in the yeast host Pichia pastoris, and a maximal laccase activity of 6.3 U mL(-1) in the fermentation broth was achieved after fermentation for 9 days. The recombinant protein rLac1 was purified, and its enzymatic properties and functional characteristics were investigated. When ABTS was used as the substrate, the enzyme was most active at pH 3.5 and 55°C and stable at pH 4-10 and 20-60°C. The Km and kcat values of rLac1 towards ABTS were 28.9μM and 332.4 s(-1), respectively. Furthermore, rLac1 was tolerant to common metal ions up to 100mM concentration and capable of decolorizing structurally different dyes in the absence of a redox mediator. Hence Lac1 may be useful for industrial applications, such as dye decolorization and bioremediation. |
| Links |
PubMed Online version:10.1016/j.ijbiomac.2015.03.028 |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016682: oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor |
ECO:0000314: |
F |
Figure 2 shows the relative activity of Lac1 on ABTS under different temperatures and pH values. Figure 3 shows the ability of Lac1 to decolorize dyes. |
complete | ||||
|
enables |
GO:0016682: oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
