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PMID:25781476

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Citation

Tripathi, D, Raikhy, G, Goodin, MM, Dietzgen, RG and Pappu, HR (2015) In Vivo Localization of Iris yellow spot Tospovirus (Bunyaviridae)-Encoded Proteins and Identification of Interacting Regions of Nucleocapsid and Movement Proteins. PLoS ONE 10:e0118973

Abstract

Localization and interaction studies of viral proteins provide important information about their replication in their host plants. Tospoviruses (Family Bunyaviridae) are economically important viruses affecting numerous field and horticultural crops. Iris yellow spot virus (IYSV), one of the tospoviruses, has recently emerged as an important viral pathogen of Allium spp. in many parts of the world. We studied the in vivo localization and interaction patterns of the IYSV proteins in uninfected and infected Nicotiana benthamiana and identified the interacting partners.

Links

PubMed Online version:10.1371/journal.pone.0118973

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

9VIRU:Q8V6X2

GO:0044538: host cell periphery

ECO:0000314:

C

IYSV NSm protein localized in the cell periphery and colocalized with the ER-RFP marker. Figure 2 E-H

complete
CACAO 10783

9VIRU:Q8V6X2

part_of

GO:0044538: host cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

9VIRU:I7CAR3

part_of

GO:0044538: host cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

9VIRU:I7CAR3

GO:0044538: host cell periphery

ECO:0000314:

C

IYSV N protein is localized in the cell periphery when expressed with a binary ER-RFP marker. Figure 2 A-D

complete
CACAO 10781

9VIRU:I7CAR3

GO:0044165: host cell endoplasmic reticulum

ECO:0000314:

C

IYSV N protein is co-localized with the endoplasmic reticulum (ER) when expressed with a binary ER-RFP marker Figure 2 A-D

complete
CACAO 10782

9VIRU:I7CAR3

part_of

GO:0044165: host cell endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

9VIRU:O40907

GO:0044538: host cell periphery

ECO:0000314:

C

IYSV NSs protein is localized in the cell periphery and colocalized with the ER-RFP marker. Figure 2 i-l

complete
CACAO 10784

9VIRU:O40907

part_of

GO:0044538: host cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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