GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:25500905
| Citation |
Lin, CC, Seikowski, J, Pérez-Lara, A, Jahn, R, Höbartner, C and Walla, PJ (2014) Control of membrane gaps by synaptotagmin-Ca(2+) measured with a novel membrane distance ruler. Nat Commun 5:5859 |
|---|---|
| Abstract |
Fast synchronous neurotransmitter release is triggered by calcium that activates synaptotagmin-1 (syt-1), resulting in fusion of synaptic vesicles with the presynaptic membrane. Syt-1 possesses two Ca(2+)-binding C2 domains that tether membranes via interactions with anionic phospholipids. It is capable of crosslinking membranes and has recently been speculated to trigger fusion by decreasing the gap between them. As quantitative information on membrane gaps is key to understanding general cellular mechanisms, including the role of syt-1, we developed a fluorescence-lifetime based inter-membrane distance ruler using membrane-anchored DNAs of various lengths as calibration standards. Wild-type and mutant data provide evidence that full-length syt-1 indeed regulates membrane gaps: without Ca(2+), syt-1 maintains membranes at distances of ~7-8 nm. Activation with 100 μM Ca(2+) decreases the distance to ~5 nm by binding the C2 domains to opposing membranes, respectively. These values reveal that activated syt-1 adjusts membrane distances to the level that promotes SNARE complex assembly. |
| Links |
PubMed PMC4275583 Online version:10.1038/ncomms6859 |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
Notes
See also
References
See Help:References for how to manage references in GONUTS.
