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PMID:2527846
| Citation |
Inada, T, Kawakami, K, Chen, SM, Takiff, HE, Court, DL and Nakamura, Y (1989) Temperature-sensitive lethal mutant of era, a G protein in Escherichia coli. J. Bacteriol. 171:5017-24 |
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| Abstract |
The era gene of Escherichia coli encodes a GTP-binding protein which has similarities to elongation factor Tu and the Saccharomyces cerevisiae RAS protein. To investigate its function, mutations affecting era were isolated. A mini-Tn10 insertion, which truncated 22 amino acids from the COOH end of Era, did not affect cell growth. By using this mini-Tn10 insert as a coselectable marker, a temperature-sensitive lethal era mutant was isolated by localized mutagenesis using P1 phage transduction. A single-base G to A change was found at position 23, causing a tyrosine residue to be substituted for the cysteine residue at position 8 (era-770), in addition to the COOH-terminal mini-Tn10 disruption. Both alterations were necessary for the temperature-sensitive phenotype. Purified Era-770 mutant protein exhibited reduced binding to GTP compared with that of the wild-type Era protein. |
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| Keywords |
Amino Acid Sequence; Bacteriophage lambda/genetics; Base Sequence; Chromosomes, Bacterial; Escherichia coli/genetics; GTP-Binding Proteins/genetics; Genes; Genes, Bacterial; Genes, Lethal; Genetic Complementation Test; Genotype; Molecular Sequence Data; Mutation; Phenotype; Plasmids; Restriction Mapping; Temperature; Transduction, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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See also
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