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PMID:24965475
| Citation |
Li, F, Sewald, X, Jin, J, Sherer, NM and Mothes, W (2014) Murine leukemia virus Gag localizes to the uropod of migrating primary lymphocytes. J. Virol. 88:10541-55 |
|---|---|
| Abstract |
B and CD4(+) T lymphocytes are natural targets of murine leukemia virus (MLV). Migrating lymphocytes adopt a polarized morphology with a trailing edge designated the uropod. Here, we demonstrate that MLV Gag localizes to the uropod in polarized B cells and CD4(+) T cells. The uropod localization of MLV Gag was dependent on plasma membrane (PM) association and multimerization of Gag but independent of the viral glycoprotein Env. Basic residues in MA that are required for MLV Gag recruitment to virological synapses between HEK293 and XC cells were dispensable for uropod localization in migrating B cells. Ultrastructural studies indicated that both wild-type and basic-residue mutant Gag localized to the outer surface of the PM at the uropod. Late-domain mutant virus particles were seen at the uropod in form of budding-arrested intermediates. Finally, uropods mediated contact between MLV-infected B cells and uninfected T cells to form virological synapses. Our results suggest that MLV, not unlike HIV, accumulates at the uropod of primary lymphocytes to facilitate viral spreading through the formation of uropod-mediated cell-cell contacts. |
| Links |
PubMed PMC4178845 Online version:10.1128/JVI.01104-14 |
| Keywords |
Animals; B-Lymphocytes/cytology; B-Lymphocytes/virology; Cell Membrane/virology; Cell Movement; Cell Polarity; Cells, Cultured; Friend murine leukemia virus/genetics; Friend murine leukemia virus/metabolism; Gene Products, gag/genetics; Gene Products, gag/metabolism; Mice; Protein Transport; Retroviridae Infections/physiopathology; Retroviridae Infections/veterinary; Retroviridae Infections/virology; Rodent Diseases/physiopathology; Rodent Diseases/virology; T-Lymphocytes/cytology; T-Lymphocytes/virology |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0120026: host cell uropod |
ECO:0000314: |
C |
Organism: Moloney murine leukemia virus protein: Gag polyprotein Figure 1: MLV Gag localizes to the uropod 1B: primary B cells (B) were infected with F-MLV Gag-GFP (green). Sixteen to 20 h after infection, cells were plated on ICAM-1-coated coverslips, immunostained for the uropod marker |
complete | ||||
Notes
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