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PMID:24686111
| Citation |
'Kim, NH, Kim, DS, Chung, EH and Hwang, BK (2014) The Pepper SGT1 Interacts with the Receptor-Like Cytoplasmic Kinase 1 and AvrBsT and Promotes Hypersensitive Cell Death Response in a Phosphorylation-Dependent Manner. Plant Physiol. ' |
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| Abstract |
Xanthomonas campestris pv. vesicatoria type III effector protein, AvrBsT, triggers hypersensitive cell death in pepper (Capsicum annuum). Here, we have identified the pepper SGT1 (suppressor of the G2 allele of skp1) as a host interactor of AvrBsT and also the pepper PIK1 (receptor-like cytoplasmic kinase 1). PIK1 specifically phosphorylates SGT1 and AvrBsT in vitro. AvrBsT specifically binds to the CS domain of SGT1, resulting in the inhibition of PIK1-mediated SGT1 phosphorylation and subsequent nuclear transport of the SGT1-PIK1 complex. Liquid chromatography-tandom mass spectrometry (LC/MS/MS) of the proteolytic peptides of SGT1 identified the residues Serine 98 and Serine 279 of SGT1 as the major PIK1-mediated phosphorylation sites. Site directed mutagenesis of SGT1 revealed that the identified SGT1 phosphorylation sites are responsible for the activation of AvrBsT-triggered cell death in planta. SGT1 forms a heterotrimeric complex with both AvrBsT and PIK1 exclusively in the cytoplasm. Agrobacterium-mediated co-expression of SGT1 and PIK1 with avrBsT promotes avrBsT-triggered cell death in Nicotiana benthamiana, dependent on PIK1. Virus-induced silencing of SGT1 and/or PIK1 compromises avrBsT-triggered cell death, H2O2 production, defense gene induction and salicylic acid accumulation, leading to the enhanced bacterial pathogen growth in pepper. Together, these results suggest that SGT1 interacts with PIK1 and bacterial effector protein AvrBsT and promotes hypersensitive cell death associated with PIK1-mediated phosphorylation in plants. |
| Links |
PubMed Online version:10.1104/pp.114.238840 |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004674: protein serine/threonine kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004674: protein serine/threonine kinase activity |
ECO:0000314: |
F |
Figure 5 C): assay showing the detection of anti-phospho-serine antibody with wild type and mutated SGT1 and PIK1 genes. Phosphorylation is observed only with WT PIK1 genes. Phosphorylation occurs on WT SGT1 and on the SGT1 mutated in the 161 serine. Other mutated serines unable to be phosphorylated by PIK1 indicate active residues. |
complete | ||||
See also
References
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