PMID:24682461

Citation	'Li, XL, Yang, X, Hu, YX, Yu, XD and Li, QL (2014) A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress tolerance. Plant Cell Rep. '
Abstract	Sl NAC1 functions as a stress-responsive NAC protein involved in the abscisic acid-dependent signaling pathway and enhances transgenic Arabidopsis drought, salt, and cold stress tolerance. NAC (NAM, ATAF1, 2, CUC2) transcription factors constitute the largest families of plant-specific transcription factors, known to be involved in various growth or developmental processes and in regulation of response to environmental stresses. However, only little information regarding stress-related NAC genes is available in Suaeda liaotungensis K. In this study, we cloned a full-length NAC gene (1,011 bp) named SlNAC1 using polymerase chain reaction from Suaeda liaotungensis K. and investigated its function by overexpression in transgenic Arabidopsis. SlNAC1 contains an NAC-conserved domain. Its expression in S. liaotungensis was induced by drought, high-salt, and cold (4 °C) stresses and by abscisic acid. Subcellular localization experiments in onion epidermal cells indicated that SlNAC1 is localized in the nucleus. Yeast one-hybrid assays showed that SlNAC1 functions as a transcriptional activator. SlNAC1 transgenic Arabidopsis displayed a higher survival ratio and lower rate of water loss under drought stress; a higher germination ratio, higher survival ratio, and lower root inhibition rate under salt stress; a higher survival ratio under cold stress; and a lower germination ratio and root inhibition rate under abscisic acid treatment, compared with wild-type Arabidopsis. These results suggested that SlNAC1 functions as a stress-responsive NAC protein involved in the abscisic acid-dependent signaling pathway and may have potential applications in transgenic breeding to enhance crops' abiotic stress tolerances.
Links	PubMed Online version:10.1007/s00299-014-1602-y
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CARY:V9P4U0		GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315:		P		Fig 4: Uses a yeast-one-hybrid screen to reveal that only transformants containing the slNAC1 gene were able to grow on a medium without His, Trp or Ade. Furthermore, only these transformants showed beta-galactosidase activity indicating transcription activation. To further support these results, Fig 3 uses a GFP protein construct to show that slNAC1 is localized in the nucleus.	complete
9CARY:V9P4U0		GO:0009819: drought recovery	ECO:0000315:		P		Figure 2: Shows both that slNAC1 is expressed more in the leaves than other parts of the plant (more susceptible to desiccation) and that it is expressed more under drought conditions. Figure 5: Shows that slNAC1 expressing plants recovered from drought and continued to grow and produce seeds while non-slNAC1 expressing plants generally died.	complete CACAO 9747
9CARY:V9P4U0	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
9CARY:V9P4U0	involved_in	GO:0009819: drought recovery	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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