PMID:24304930

Citation	Sun, L, Andika, IB, Shen, J, Yang, D and Chen, J (2014) The P2 of Wheat yellow mosaic virus rearranges the endoplasmic reticulum and recruits other viral proteins into replication-associated inclusion bodies. Mol. Plant Pathol. 15:466-78
Abstract	Viruses commonly modify host endomembranes to facilitate biological processes in the viral life cycle. Infection by viruses belonging to the genus Bymovirus (family Potyviridae) has long been known to induce the formation of large membranous inclusion bodies in host cells, but their assembly and biological roles are still unclear. Immunoelectron microscopy of cells infected with the bymovirus Wheat yellow mosaic virus (WYMV) showed that P1, P2 and P3 are the major viral protein constituents of the membranous inclusions, whereas NIa-Pro (nuclear inclusion-a protease) and VPg (viral protein genome-linked) are probable minor components. P1, P2 and P3 associated with the endoplasmic reticulum (ER), but only P2 was able to rearrange ER and form large aggregate structures. Bioinformatic analyses and chemical experiments showed that P2 is an integral membrane protein and depends on the active secretory pathway to form aggregates of ER membranes. In planta and in vitro assays demonstrated that P2 interacts with P1, P3, NIa-Pro or VPg and recruits these proteins into the aggregates. In vivo RNA labelling using WYMV-infected wheat protoplasts showed that the synthesis of viral RNAs occurs in the P2-associated inclusions. Our results suggest that P2 plays a major role in the formation of membranous compartments that house the genomic replication of WYMV.
Links	PubMed Online version:10.1111/mpp.12109
Keywords	Endoplasmic Reticulum/metabolism; Inclusion Bodies/metabolism; RNA, Viral/genetics; Triticum/virology; Viral Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9POTY:Q9IWA2		GO:0044165: host cell endoplasmic reticulum	ECO:0000314:		C		WYMV RNA2 Polyprotein (P1 & P2) “RNA2 (∼3.5 kb) encodes a polyprotein that is cleaved into two proteins, P1 and P2 (Namba et al., 1998) (see also Fig. 2A).” // “P1-eGFP labelled the tubular ER network, but more strongly accumulated in numerous ring-like structures located at the vertices of the connecting ER tubules… P2-eGFP overlapped with the ER marker at these structures (Fig. 3B).”	complete CACAO 12705
9POTY:Q9IWA2		GO:0052043: modification by symbiont of host cellular component	ECO:0000314:		P		WYMV (Wheat Yellow Mosaic Virus) RNA2 Polyprotein (P2) “RNA2 (∼3.5 kb) encodes a polyprotein that is cleaved into two proteins, P1 and P2 (Namba et al., 1998) (see also Fig. 2A).” // “Notably, cortical ER networks were much less visible in the areas surrounding aggregates, suggesting that P2 rearranges tubular ER into aggregates. Time-lapse imaging demonstrated that some of the aggregates were mobile and merged with other aggregates (Fig. 3C).”	complete CACAO 12706
9POTY:Q9IWA2		GO:0019060: intracellular transport of viral protein in host cell	ECO:0000314:		P		WYMV RNA2 Polyprotein (P2) “RNA2 (∼3.5 kb) encodes a polyprotein that is cleaved into two proteins, P1 and P2 (Namba et al., 1998) (see also Fig. 2A).” // “CLSM observation showed that P1-, P3-, VPg- and NIa-Ppaper shows that an active secretory pathway is required (Fig 5) and data not shown.	complete CACAO 12734
9POTY:X5ICX9		GO:0044165: host cell endoplasmic reticulum	ECO:0000314:		C		WYMV Polyprotein (P3) “WYMV RNA1 (∼7.6 kb) encodes a large polyprotein that is proteolytically processed to generate eight mature proteins, namely P3” // “P3-eGFP accumulated in the perinuclear and cortical ER and also localized in small mobile vesicular bodies… Thus, unlike P1 and P2, P3 targets both the ER and the Golgi apparatus,” (Fig 3B).	complete CACAO 12731
9POTY:X5ICX9		GO:0044177: host cell Golgi apparatus	ECO:0000314:		C		WYMV Polyprotein (P3) “WYMV RNA1 (∼7.6 kb) encodes a large polyprotein that is proteolytically processed to generate eight mature proteins, namely P3” // “When P3-eGFP was co-expressed with a Golgi-specific marker (ManI-Cherry), these small vesicular bodies overlapped with Golgi stacks (Fig. 3B). Thus, unlike P1 and P2, P3 targets both the ER and the Golgi apparatus.”	complete CACAO 12732
9POTY:X5ICX9		GO:0032541: cortical endoplasmic reticulum	ECO:0000314:		C		WYMV Polyprotein (7K) “RNA1 (∼7.6 kb) encodes a large polyprotein that is proteolytically processed to generate eight mature proteins, namely P3, 7K, CI (cylindrical inclusion), 14K, VPg (viral protein genome-linked), NIa-Pro (nuclear inclusion-a protease), NIb and CP (coat protein)” // “7K fused to eGFP (7K-eGFP) localized to the cortical ER network (Fig. S1, see Supporting Information).”	complete CACAO 12733

References

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