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PMID:24291253
| Citation |
Karttunen, J, Mäntynen, S, Ihalainen, TO, Lehtivuori, H, Tkachenko, NV, Vihinen-Ranta, M, Ihalainen, JA, Bamford, JK and Oksanen, HM (2014) Subcellular localization of bacteriophage PRD1 proteins in Escherichia coli. Virus Res. 179:44-52 |
|---|---|
| Abstract |
Bacteria possess an intricate internal organization resembling that of the eukaryotes. The complexity is especially prominent at the bacterial cell poles, which are also known to be the preferable sites for some bacteriophages to infect. Bacteriophage PRD1 is a well-known model serving as an ideal system to study structures and functions of icosahedral internal membrane-containing viruses. Our aim was to analyze the localization and interactions of individual PRD1 proteins in its native host Escherichia coli. This was accomplished by constructing a vector library for production of fluorescent fusion proteins. Analysis of solubility and multimericity of the fusion proteins, as well as their localization in living cells by confocal microscopy, indicated that multimeric PRD1 proteins were prone to localize in the cell poles. Furthermore, PRD1 spike complex proteins P5 and P31, as fusion proteins, were shown to be functional in the virion assembly. In addition, they were shown to co-localize in the specific polar area of the cells, which might have a role in the multimerization and formation of viral protein complexes. |
| Links |
PubMed Online version:10.1016/j.virusres.2013.11.015 |
| Keywords |
Bacteriophage PRD1/genetics; Bacteriophage PRD1/metabolism; Escherichia coli/virology; Intracellular Space/virology; Protein Transport; Viral Proteins/genetics; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0060187: cell pole |
ECO:0007107: yellow fluorescent protein fusion protein localization evidence used in manual assertion |
C |
Fig. 3 shows eYFP-P5 protein fusions localize at cell poles. UniProtKB: P22536 (P5_BPPRD) Organism: Enterobacteria phage PRD1 (Bacteriophage PRD1) UniProt submitted name: Spike protein P5 |
complete | ||||
| GO:0060187: cell pole |
ECO:0007107: yellow fluorescent protein fusion protein localization evidence used in manual assertion |
C |
Fig. 3 shows that eYFP-P31 protein fusion localizes at the cell pole. UniProtKB: P27384 (VP31_BPPRD) Organism: Enterobacteria phage PRD1 (Bacteriophage PRD1) UniProt submitted name: Penton protein P31 |
complete | ||||
Notes
See also
References
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