PMID:24191970

Citation	Studer, SV, Schwartzman, JA, Ho, JS, Geske, GD, Blackwell, HE and Ruby, EG (2014) Non-native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stability. Environ. Microbiol. 16:2623-34
Abstract	Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR-type N-acyl L-homoserine (AHL) quorum sensing is common in Gram-negative Proteobacteria, and many members of this group have additional quorum-sensing networks. The bioluminescent symbiont Vibrio fischeri encodes two AHL signal synthases: AinS and LuxI. AinS-dependent quorum sensing converges with LuxI-dependent quorum sensing at the LuxR regulatory element. Both AinS- and LuxI-mediated signalling are required for efficient and persistent colonization of the squid host, Euprymna scolopes. The basis of the mutualism is symbiont bioluminescence, which is regulated by both LuxI- and AinS-dependent quorum sensing, and is essential for maintaining a colonization of the host. Here, we used chemical and genetic approaches to probe the dynamics of LuxI- and AinS-mediated regulation of bioluminescence during symbiosis. We demonstrate that both native AHLs and non-native AHL analogues can be used to non-invasively and specifically modulate induction of symbiotic bioluminescence via LuxI-dependent quorum sensing. Our data suggest that the first day of colonization, during which symbiont bioluminescence is induced by LuxIR, is a critical period that determines the stability of the V. fischeri population once symbiosis is established.
Links	PubMed PMC4010582 Online version:10.1111/1462-2920.12322
Keywords	4-Butyrolactone/analogs & derivatives; 4-Butyrolactone/metabolism; Aliivibrio fischeri/genetics; Aliivibrio fischeri/metabolism; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Decapodiformes/microbiology; Gene Expression Regulation, Bacterial; Luminescence; Quorum Sensing/genetics; Repressor Proteins/genetics; Repressor Proteins/metabolism; Symbiosis/genetics; Time Factors; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism
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