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PMID:23992865
| Citation |
Yu, D, Xu, F, Zhang, S, Shao, L, Wang, S and Zhan, J (2013) Characterization of a methyltransferase involved in herboxidiene biosynthesis. Bioorg. Med. Chem. Lett. 23:5667-70 |
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| Abstract |
The herboxidiene biosynthetic gene cluster contains a regulatory gene and six biosynthetic genes that encode three polyketide synthases (HerB, HerC and HerD) and three tailoring enzymes (HerE, HerF and HerG). Through single crossover recombination, an integrative plasmid was inserted into the genome of Streptomyces chromofuscus ATCC 49982 between herE and herF, resulting in low-level expression of herF and the downstream herG. The mutant strain produced two new compounds, 18-deoxy-25-demethyl-herboxidiene and 25-demethyl-herboxidiene. HerF was expressed in Escherichia coli and biochemically characterized as the dedicated methyltransferase in herboxidiene biosynthesis. It prefers 25-demethyl-herboxidiene to 18-deoxy-25-demethyl-herboxidiene, suggesting that C-25 methylation is the last tailoring step. |
| Links |
PubMed Online version:10.1016/j.bmcl.2013.08.023 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0008168: methyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0008168: methyltransferase activity |
ECO:0000314: |
F |
Figure 4 shows the methylation activity of HerF in the production of herboxidiene |
complete | ||||
Notes
See also
References
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