PMID:23964570

Citation	Yang, L, Xu, L, Li, Y, Li, J, Bi, Y and Liu, W (2013) Molecular and functional characterization of canine interferon-epsilon. J. Interferon Cytokine Res. 33:760-8
Abstract	In this study, we provide the first comprehensive annotation of the entire family of canine interferons (IFNs). Canine IFN-ε (IFNE), IFN-κ (IFNK), and IFN-λ (IFNL) were discovered for the first time. Ten functional and 2 truncated IFN-α (IFNA) pseudogenes were found in the genome, which also enriched the existing knowledge about canine IFNA. The canine type I IFN genes are clustered on chromosome 11, and their relative arrangements are illustrated. To further investigate the biological activity of canine IFNE, it was expressed and purified in Escherichia coli. Recombinant canine IFNE (rCaIFN-ε) displayed potent antiviral activity on both homologous and heterologous animal cells in vitro, indicating that rCaIFN-ε has more broad cross-species activity than recombinant canine IFNA (rCaIFN-α). The antiviral activities of rCaIFN-ε and rCaIFN-α7 against different viruses on MDCK cells were also evaluated. The antiviral activities of recombinant canine IFNK and IFNL were demonstrated using a VSV-MDCK virus-target cell system. rCaIFN-ε exhibited a significant anti-proliferative response against A72 canine tumor cells and MDCK canine epithelial cells in a dose-dependent manner. rCaIFN-α7 was approximately 16-fold more potent than rCaIFN-ε in promoting natural killer cell cytotoxicity activity. Further, rCaIFN-ε can activate the JAK-STAT signaling pathway.
Links	PubMed PMC3868301 Online version:10.1089/jir.2013.0037
Keywords	Animals; Antiviral Agents/pharmacology; Cell Line; Cell Proliferation/drug effects; Chromosome Mapping; Cloning, Molecular; Dogs; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Genetic Loci; Genome; Interferons/genetics; Interferons/metabolism; Interferons/pharmacology; Janus Kinases/metabolism; Killer Cells, Natural/drug effects; Killer Cells, Natural/immunology; Phylogeny; STAT Transcription Factors/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Signal Transduction/drug effects; Viruses/drug effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CANLF:R9XMN7		GO:0008285: negative regulation of cell proliferation	ECO:0000314:		P		Figure 4	complete CACAO 10256
CANLF:R9XMN7		GO:0046427: positive regulation of JAK-STAT cascade	ECO:0000314:		P		Figure 6	complete CACAO 10257
CANLF:R9XMN7		GO:0030101: natural killer cell activation	ECO:0000314:		P		Figure 5	complete CACAO 10331
CANLF:R9XMN7	involved_in	GO:0008285: negative regulation of cell population proliferation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CANLF:R9XMN7	involved_in	GO:0046427: positive regulation of receptor signaling pathway via JAK-STAT	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CANLF:R9XMN7	involved_in	GO:0030101: natural killer cell activation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CANLF:Q75UL5		GO:0030101: natural killer cell activation	ECO:0000314:		P		Figure 5	complete CACAO 10334
CANLF:Q75UL5	involved_in	GO:0030101: natural killer cell activation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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