GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:23868295
| Citation |
Xiao, J, Zhang, Q, Zhu, Y, Li, S, Zhang, G, Zhang, H, Saurav, K and Zhang, C (2013) Characterization of the sugar-O-methyltransferase LobS1 in lobophorin biosynthesis. Appl. Microbiol. Biotechnol. 97:9043-53 |
|---|---|
| Abstract |
Lobophorins A (1) and B (2) belong to a large group of spirotetronate natural products with potent antibacterial and antitumor activities. The cloning of the lobophorin biosynthesis gene cluster from the deep-sea-derived Streptomyces sp. SCSIO 01127 identified a sugar-O-methyltransferase-encoding gene lobS1. The lobS1 inactivation mutant accumulated two new lobophorin analogs 3 and 4, different from 1 and 2 by lacking the 4-methyl group at the terminal L-digitoxose, respectively. Biochemical experiments verified that LobS1 was a SAM-dependent sugar-O-methyltransferase that required divalent metal ions for better activity. Antibacterial assays revealed compounds 3 and 4 were generally less potent than compounds 1 and 2. These findings suggest that the methylation on the terminal digitoxose by LobS1 tailors lobophorin biosynthesis and highlights the importance of this methylation for antibacterial potence. |
| Links |
PubMed Online version:10.1007/s00253-013-5083-7 |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008171: O-methyltransferase activity |
ECO:0000315: |
F |
Figure 2 |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
