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PMID:23700350
Citation |
Watanabe, S, Hanaoka, M, Ohba, Y, Ono, T, Ohnuma, M, Yoshikawa, H, Taketani, S and Tanaka, K' (2013) Mitochondrial localization of ferrochelatase in a red alga Cyanidioschyzon merolae. Plant Cell Physiol. ' |
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Abstract |
Ferrochelatase (FECH) is an essential enzyme for the final step of heme biosynthesis. In green plants, its activity has been reported in both plastids and mitochondria. However, the precise subcellular localization of FECH remains uncertain. In this study, we analyzed the localization of FECH in the unicellular red alga, Cyanidioschyzon merolae. Immunoblot and enzyme activity analyses of subcellular fractions localized little FECH in the plastid. In addition, immunofluorescence microscopy identified that both intrinsic and HA-tagged FECH are localized in the mitochondrion. We therefore conclude that FECH is localized in the mitochondrion in C. merolae. |
Links |
PubMed Online version:10.1093/pcp/pct077 |
Keywords |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005739: mitochondrion |
ECO:0000314: |
C |
Figure 4G-J |
complete | ||||
part_of |
GO:0005739: mitochondrion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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