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PMID:23700350

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Citation

Watanabe, S, Hanaoka, M, Ohba, Y, Ono, T, Ohnuma, M, Yoshikawa, H, Taketani, S and Tanaka, K' (2013) Mitochondrial localization of ferrochelatase in a red alga Cyanidioschyzon merolae. Plant Cell Physiol. '

Abstract

Ferrochelatase (FECH) is an essential enzyme for the final step of heme biosynthesis. In green plants, its activity has been reported in both plastids and mitochondria. However, the precise subcellular localization of FECH remains uncertain. In this study, we analyzed the localization of FECH in the unicellular red alga, Cyanidioschyzon merolae. Immunoblot and enzyme activity analyses of subcellular fractions localized little FECH in the plastid. In addition, immunofluorescence microscopy identified that both intrinsic and HA-tagged FECH are localized in the mitochondrion. We therefore conclude that FECH is localized in the mitochondrion in C. merolae.

Links

PubMed Online version:10.1093/pcp/pct077

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CYAM1:M1VBI3

GO:0005739: mitochondrion

ECO:0000314:

C

Figure 4G-J

complete
CACAO 8497

CYAM1:M1VBI3

part_of

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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