PMID:23649177

Citation	Lee, JH, Shin, H, Son, B, Heu, S and Ryu, S (2013) Characterization and complete genome sequence of a virulent bacteriophage B4 infecting food-borne pathogenic Bacillus cereus. Arch. Virol. 158:2101-8
Abstract	Bacillus cereus causes food poisoning, resulting in vomiting and diarrhea, due to production of enterotoxins. As a means of controlling this food-borne pathogen, the virulent bacteriophage B4 was isolated and characterized. Bacterial challenge assays showed that phage B4 effectively inhibited growth of members of the B. cereus group as well as B. subtilis, and growth inhibition persisted for over 20 h. One-step growth analysis also revealed the host lysis activity of phage B4, with relatively short eclipse/latent times (10/15 min) and a large burst size (>200 PFU). The complete genome of phage B4, containing a 162-kb DNA with 277 ORFs, was analyzed. The endolysin encoded by the phage B4 genome accounts for the cell lysis activity of this phage. These results suggest that phage B4 has potential as a biological agent to control B. cereus propagation.
Links	PubMed Online version:10.1007/s00705-013-1719-2
Keywords	Bacillus cereus/virology; Bacteriophages/genetics; Bacteriophages/metabolism; Bacteriophages/pathogenicity; Gene Expression Regulation, Viral/physiology; Genome, Viral; Molecular Sequence Data; Phylogeny; Viral Proteins/genetics; Viral Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:J9PUX9		GO:0098015: virus tail	ECO:0000250:	UniProtKB:M4HPF1	C		In paper table 2 shows BCB4_0238 is putative tail protein.Blast shows BCB4_0238 of Bacillus phage B4 is homolog with orf203 of Bc431v3. E-value is 0.0, query cover is 99%. orf203 of is tail protein.	complete CACAO 11665

References

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