PMID:23606412

Citation	Luo, X, Bai, X, Sun, X, Zhu, D, Liu, B, Ji, W, Cai, H, Cao, L, Wu, J, Hu, M, Liu, X, Tang, L and Zhu, Y (2013) Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling. J. Exp. Bot. 64:2155-69
Abstract	The WRKY-type transcription factors are involved in plant development and stress responses, but how the regulation of stress tolerance is related to plant development is largely unknown. GsWRKY20 was initially identified as a stress response gene using large-scale Glycine soja microarrays. Quantitative reverse transcription-PCR (qRT-PCR) showed that the expression of this gene was induced by abscisic acid (ABA), salt, cold, and drought. Overexpression of GsWRKY20 in Arabidopsis resulted in a decreased sensitivity to ABA during seed germination and early seedling growth. However, compared with the wild type, GsWRKY20 overexpression lines were more sensitive to ABA in stomatal closure, and exhibited a greater tolerance to drought stress, a decreased water loss rate, and a decreased stomatal density. Moreover, microarray and qRT-PCR assays showed that GsWRKY20 mediated ABA signalling by promoting the expression of negative regulators of ABA signalling, such as AtWRKY40, ABI1, and ABI2, while repressing the expression of the positive regulators of ABA, for example ABI5, ABI4, and ABF4. Interestingly, GsWRKY20 also positively regulates the expression of a group of wax biosynthetic genes. Further, evidence is provided to support that GsWRKY20 overexpression lines have more epicuticular wax crystals and a much thicker cuticle, which contribute to less chlorophyll leaching compared with the wild type. Taken together, the findings reveal an important role for GsWRKY20 in enhancing drought tolerance and regulating ABA signalling.
Links	PubMed Online version:10.1093/jxb/ert073
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SOYBN:A7LHG5	involved_in	GO:0009737: response to abscisic acid	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
SOYBN:A7LHG5		GO:0009737: response to abscisic acid stimulus	ECO:0000315:		P		Figure 3D illustrates that three transgenic Arabidopsis lines with overexpression of GsWRKY20 were able to germinate much faster than wild type when exposed to 0.8 μM ABA. Figure 3F shows that overexpressing GsWRKY20 Arabidopsis lines had significantly higher percentages of leaf opening and greening at different ABA concentrations than wild type. Fig 8	complete CACAO 8457

References

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