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PMID:23516598
| Citation |
de Melo, IS, Jimenez-Nuñez, MD, Iglesias, C, Campos-Caro, A, Moreno-Sanchez, D, Ruiz, FA and Bolívar, J (2013) NOA36 Protein Contains a Highly Conserved Nucleolar Localization Signal Capable of Directing Functional Proteins to the Nucleolus, in Mammalian Cells. PLoS ONE 8:e59065 |
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| Abstract |
NOA36/ZNF330 is an evolutionarily well-preserved protein present in the nucleolus and mitochondria of mammalian cells. We have previously reported that the pro-apoptotic activity of this protein is mediated by a characteristic cysteine-rich domain. We now demonstrate that the nucleolar localization of NOA36 is due to a highly-conserved nucleolar localization signal (NoLS) present in residues 1-33. This NoLS is a sequence containing three clusters of two or three basic amino acids. We fused the amino terminal of NOA36 to eGFP in order to characterize this putative NoLS. We show that a cluster of three lysine residues at positions 3 to 5 within this sequence is critical for the nucleolar localization. We also demonstrate that the sequence as found in human is capable of directing eGFP to the nucleolus in several mammal, fish and insect cells. Moreover, this NoLS is capable of specifically directing the cytosolic yeast enzyme polyphosphatase to the target of the nucleolus of HeLa cells, wherein its enzymatic activity was detected. This NoLS could therefore serve as a very useful tool as a nucleolar marker and for directing particular proteins to the nucleolus in distant animal species. |
| Links |
PubMed PMC3596294 Online version:10.1371/journal.pone.0059065 |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008104: protein localization |
ECO:0000314: |
P |
The nucleolar localization of Flag-NOA36 recombinant protein was confirmed using an anti-UBF antibody as nucleolar marker(Fig 2C. The nucleolar staining was not homogeneous, being localized mainly on the periphery of the nucleolus. From this data it is concluded that NOA36 contains at least one nucleolar localization signal. |
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See also
References
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