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PMID:23516427
| Citation |
Yurgel, SN, Rice, J and Kahn, ML (2013) Transcriptome analysis of the role of GlnD/GlnBK in nitrogen stress adaptation by Sinorhizobium meliloti Rm1021. PLoS ONE 8:e58028 |
|---|---|
| Abstract |
Transcriptional changes in the nitrogen stress response (NSR) of wild type S. meliloti Rm1021, and isogenic strains missing both PII proteins, GlnB and GlnK, or carrying a ΔglnD-sm2 mutation were analyzed using whole-genome microarrays. This approach allowed us to identify a number of new genes involved in the NSR and showed that the response of these bacteria to nitrogen stress overlaps with other stress responses, including induction of the fixK2 transcriptional activator and genes that are part of the phosphate stress response. Our data also show that GlnD and GlnBK proteins may regulate many genes that are not part of the NSR. Analysis of transcriptome profiles of the Rm1021 ΔglnD-sm2 strain allowed us to identify several genes that appear to be regulated by GlnD without the participation of the PII proteins. |
| Links |
PubMed PMC3596328 Online version:10.1371/journal.pone.0058028 |
| Keywords |
Adaptation, Biological; Bacterial Proteins/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Models, Biological; Molecular Sequence Annotation; Mutation; Nitrogen/metabolism; PII Nitrogen Regulatory Proteins/genetics; PII Nitrogen Regulatory Proteins/metabolism; Phosphates/metabolism; Polysaccharides, Bacterial/biosynthesis; Reproducibility of Results; Sinorhizobium meliloti/genetics; Sinorhizobium meliloti/metabolism; Stress, Physiological |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0010468: regulation of gene expression |
ECO:0000315: |
P |
Under the headline "Global Nitrogen Stress Response" you will find the information how the GlnD protein and some mutations of this protein relate to gene expression of different metabolisms and general stress responses. Figure 5 also shows the role of GlnD in general stress response through the PII protein system. |
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See also
References
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