PMID:23511337

Citation	Li, Z, Yao, F, Chen, Y, Zhang, R, Lv, Y, Zhao, N, Wang, T, Xin, W, Hou, L and Zou, X (2013) Molecular cloning, characterization and expression analysis of ubiquitin protein ligase gene (As-ubpl) from Artemia sinica. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 165:90-8
Abstract	Ubiquitylation is an important protein post-translational regulation pathway, which is involved in controlling protein degradation, tumor occurrence and cell cycle regulation. E3 ubiquitin protein ligase (UBPL) plays a crucial role of the conjugation of activated ubiquitin to protein substrates and leads to targeting proteins for degradation by the proteasome. We amplified one full-length cDNA of the A. sinica UBPL (As-ubpl) gene by RACE technology. The full-length cDNA of As-ubpl is composed of 2931 bp, with a 2571 bp open reading frame (ORF) that encodes a polypeptide of 856 amino acids with a C2 domain, two domains with two conserved Trp (W) residues (WW) and a homologous to E6-AP Carboxyl Terminus (HECT) domain. The amount of As-ubpl showed from real-time PCR indicates that a high expression levels of As-ubpl at 20 h, 40 h and 3 days of embryo development, with highest expression levels appearing in the larval stage (40 h). Furthermore, As-ubpl transcripts were highly up-regulated under salinity (50‰) and low temperature stress (15 °C). These results indicate that As-ubpl is involved in protein regulation of the postdiapause development and in responses to salinity and low temperature stress.
Links	PubMed Online version:10.1016/j.cbpb.2013.03.009
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CRUS:K7WBL1	involved_in	GO:0051438: regulation of ubiquitin-protein transferase activity	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
9CRUS:K7WBL1		GO:0004842: ubiquitin-protein ligase activity	ECO:0000316:	UniProtKB:K7WBL1	F		The article does an analysis on the regulatory pathway of ubiquitin-protein ligase, which has important roles in protein degradation.	complete CACAO 8677
9CRUS:K7WBL1		GO:0004842: ubiquitin-protein ligase activity	ECO:0000255:	UniProtKB:K7WBL1	F		Figure 1: From sequence analysis of As-UBPL and complete cDNA sequence and deduce protein sequence.	complete CACAO 8678
9CRUS:K7WBL1		GO:0051438: regulation of ubiquitin-protein ligase activity	ECO:0000314:		P		Figure 7: A PCR analysis of As-UBPL expression in salinity challenge assay.	complete CACAO 8679
9CRUS:K7WBL1		GO:0051483: terpenoid biosynthetic process, mevalonate-independent	ECO:0000314:		P		Figure 8: Quantitative PCR analysis of As-UBPL expression in temperature challenge assay.	complete CACAO 8680

References

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