PMID:23498860

Citation	Seferoglu, AB, Baris, I, Morgil, H, Tulum, I, Ozdas, S, Cevahir, G and Kavakli, IH (2013) Transcriptional regulation of the ADP-glucose pyrophosphorylase isoforms in the leaf and the stem under long and short photoperiod in lentil. Plant Sci. 205-206:29-37
Abstract	ADP-glucose pyrophosphorylase (AGPase) is a key enzyme in plant starch biosynthesis. It contains large (LS) and small (SS) subunits encoded by two different genes. In this study, we explored the transcriptional regulation of both the LS and SS subunits of AGPase in stem and leaf under different photoperiods length in lentil. To this end, we first isolated and characterized different isoforms of the LS and SS of lentil AGPase and then we performed quantitative real time PCR (qPCR) to see the effect of photoperiod length on the transcription of the AGPase isforms under the different photoperiod regimes in lentil. Analysis of the qPCR results revealed that the transcription of different isoforms of the LSs and the SSs of lentil AGPase are differentially regulated when photoperiod shifted from long-day to short-day in stem and leaves. While transcript levels of LS1 and SS2 in leaf significantly decreased, overall transcript levels of SS1 increased in short-day regime. Our results indicated that day length affects the transcription of lentil AGPase isoforms differentially in stems and leaves most likely to supply carbon from the stem to other tissues to regulate carbon metabolism under short-day conditions.
Links	PubMed Online version:10.1016/j.plantsci.2013.01.006
Keywords	Base Sequence; Cloning, Molecular; Gene Expression Regulation, Plant/genetics; Glucose-1-Phosphate Adenylyltransferase/genetics; Glucose-1-Phosphate Adenylyltransferase/isolation & purification; Glucose-1-Phosphate Adenylyltransferase/metabolism; Isoenzymes; Kinetics; Lens Plant/enzymology; Lens Plant/genetics; Lens Plant/radiation effects; Molecular Sequence Data; Organ Specificity; Photoperiod; Phylogeny; Plant Leaves/enzymology; Plant Leaves/genetics; Plant Leaves/radiation effects; Plant Proteins/genetics; Plant Proteins/isolation & purification; Plant Proteins/metabolism; Plant Roots/enzymology; Plant Roots/genetics; Plant Roots/radiation effects; Plant Stems/enzymology; Plant Stems/genetics; Plant Stems/radiation effects; Seeds/enzymology; Seeds/genetics; Seeds/radiation effects; Starch/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LENCU:D0ENL5	enables	GO:0008878: glucose-1-phosphate adenylyltransferase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
LENCU:D0ENL5		GO:0008878: glucose-1-phosphate adenylyltransferase activity	ECO:0000314:		F		Table 1	complete CACAO 8888

References

See Help:References for how to manage references in GONUTS.