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PMID:23469147
| Citation |
Vilela, B, Moreno-Cortés, A, Rabissi, A, Leung, J, Pagès, M and Lumbreras, V (2013) The maize OST1 kinase homolog phosphorylates and regulates the maize SNAC1-type transcription factor. PLoS ONE 8:e58105 |
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| Abstract |
The Arabidopsis kinase OPEN STOMATA 1 (OST1) plays a key role in regulating drought stress signalling, particularly stomatal closure. We have identified and investigated the functions of the OST1 ortholog in Z. mays (ZmOST1). Ectopic expression of ZmOST1 in the Arabidopsis ost1 mutant restores the stomatal closure phenotype in response to drought. Furthermore, we have identified the transcription factor, ZmSNAC1, which is directly phosphorylated by ZmOST1 with implications on its localization and protein stability. Interestingly, ZmSNAC1 binds to the ABA-box of ZmOST1, which is conserved in SnRK2s activated by ABA and is part of the contact site for the negative-regulating clade A PP2C phosphatases. Taken together, our results indicate that ZmSNAC1 is a substrate of ZmOST1 and delineate a novel osmotic stress transcriptional pathway in maize. |
| Links |
PubMed PMC3585266 Online version:10.1371/journal.pone.0058105 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:2000070: regulation of response to water deprivation |
ECO:0000315: |
P |
Figure 1C shows partial phenotypic ost1-2 complementation by ZmOST1 in drought signaling through comparing temperature of detached leaves by infrared thermography. |
complete | ||||
| GO:0004672: protein kinase activity |
ECO:0000315: |
F |
Figure 5B demonstrates in vitro ZmSNAC1 phosphorylation by ZmOST1. Figure 5C illustrates that kinase activity is lost in extracts from the ost1-2allele and recovered in extracts of the ZmOST1/ost1-2 complemented line (Arabidopsis). |
complete | ||||
|
enables |
GO:0004672: protein kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:2000070: regulation of response to water deprivation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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