PMID:23461641

Citation	Pasternak, C, Dulermo, R, Ton-Hoang, B, Debuchy, R, Siguier, P, Coste, G, Chandler, M and Sommer, S' (2013) ISDra2 transposition in Deinococcus radiodurans is downregulated by TnpB. Mol. Microbiol. '
Abstract	Transposable elements belonging to the recently identified IS200/IS605 family radically differ from classical insertion sequences in their transposition mechanism by strictly requiring single-stranded DNA substrates. This IS family includes elements encoding only the transposase (TnpA), and others, like ISDra2 from Deinococcus radiodurans, which contain a second gene, tnpB, dispensable for transposition and of unknown function to date. Here, we show that TnpB has an inhibitory effect on the excision and insertion steps of ISDra2 transposition. This inhibitory action of TnpB was maintained when ISDra2 transposition was induced by γ-irradiation of the host cells and required the integrity of its putative zinc finger motif. We also demonstrate the negative role of TnpB when ISDra2 transposition was monitored in a heterologous Escherichia coli host, indicating that TnpB-mediated inhibition does not involve Deinococcus-specific factors. TnpB therefore appears to play a regulatory role in ISDra2 transposition.
Links	PubMed Online version:10.1111/mmi.12194
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DEIRD:O83028		GO:0070895: negative regulation of transposon integration	ECO:0000315:		P		Figure 3B. Shows in vivo TnpB down regulation of transposon integration, in the presence of TnpA.	complete CACAO 7015
DEIRD:O83028	involved_in	GO:0070895: negative regulation of transposon integration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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