PMID:23458874

Citation	Li, HB and Du, YZ (2013) Molecular cloning and characterization of an Hsp90/70 organizing protein gene from Frankliniella occidentalis (Insecta: Thysanoptera, Thripidae). Gene 520:148-55
Abstract	The heat shock 90/70 organizing protein (Hop), also known as Sti-1 (stress-induced protein-1), is a co-chaperone that usually mediates the interaction of Hsp90 and Hsp70 and has been extensively characterized in mammals and plants. However, its role in insects remains unknown. In the present study, we isolated and characterized a Hop homologue gene from Frankliniella occidentalis (Fohop). The Fohop contains a 1659bp ORF encoding a protein of 552 amino acids with a caculated molecular mass of approximately 62.25kDa, which displays a reasonable degree of identity with the known Hops and shares several canonical motifs, including three tetratricopeptide repeated motif domains (TPR1, TPR2A and TPR2B) and two aspartic acid-proline (DP) repeat motifs (DP1 and DP2). As in other hops, Fohop contains introns, but the number and the position are quite variable. The mRNA expression patterns indicated that Fohop was constitutively expressed throughout the developmental stages, but was obviously upregulated by heat stress both in larvae and adults. Our studies imply that Hop, as in other Hsps, may play an important role in heat shock response of F. occidentalis.
Links	PubMed Online version:10.1016/j.gene.2013.02.026
Keywords	Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; HSP70 Heat-Shock Proteins/metabolism; HSP90 Heat-Shock Proteins/metabolism; Heat-Shock Proteins/chemistry; Heat-Shock Proteins/genetics; Heat-Shock Proteins/isolation & purification; Heat-Shock Proteins/metabolism; Insect Proteins/chemistry; Insect Proteins/genetics; Insect Proteins/isolation & purification; Insect Proteins/metabolism; Models, Molecular; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Molecular Sequence Data; Phylogeny; Protein Binding; Sequence Homology; Thysanoptera/genetics; Thysanoptera/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
FRAOC:K4NWS2		GO:0042026: protein refolding	ECO:0000266:	PMID:23458874	P		Figure 2 shows amino acid sequence alignments of FoHop with other insect homologues.	complete CACAO 8808
FRAOC:K4NWS2		GO:0006950: response to stress	ECO:0000266:	PMID:23458874	P		Figure 5 shows a comparison of genomic structure of Fohop with hops of other insects.	complete CACAO 8810
FRAOC:K4NWS2		GO:0006950: response to stress	ECO:0000314:		P		Figure 6 shows mRNA expression level of Fohop at different developmental stages.	complete CACAO 8811

References

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