PMID:23431288

Citation	Wu, X, Wu, J, Luo, Y, Bragg, J, Anderson, O, Vogel, J and Gu, YQ (2013) Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon. Int J Plant Genomics 2013:423189
Abstract	Caffeic acid o-methyltransferase (COMT) is one of the important enzymes controlling lignin monomer production in plant cell wall synthesis. Analysis of the genome sequence of the new grass model Brachypodium distachyon identified four COMT gene homologs, designated as BdCOMT1, BdCOMT2, BdCOMT3, and BdCOMT4. Phylogenetic analysis suggested that they belong to the COMT gene family, whereas syntenic analysis through comparisons with rice and sorghum revealed that BdCOMT4 on Chromosome 3 is the orthologous copy of the COMT genes well characterized in other grass species. The other three COMT genes are unique to Brachypodium since orthologous copies are not found in the collinear regions of rice and sorghum genomes. Expression studies indicated that all four Brachypodium COMT genes are transcribed but with distinct patterns of tissue specificity. Full-length cDNAs were cloned in frame into the pQE-T7 expression vector for the purification of recombinant Brachypodium COMT proteins. Biochemical characterization of enzyme activity and substrate specificity showed that BdCOMT4 has significant effect on a broad range of substrates with the highest preference for caffeic acid. The other three COMTs had low or no effect on these substrates, suggesting that a diversified evolution occurred on these duplicate genes that not only impacted their pattern of expression, but also altered their biochemical properties.
Links	PubMed PMC3562662 Online version:10.1155/2013/423189
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BRADI:I1I1F5		GO:0047763: caffeate O-methyltransferase activity	ECO:0000314:		F		As seen in table 1, the BdCOMT4 recombinant protein displayed strong caffeate O-methyltransferase activity when utilizing caffeic acid as a substrate.	complete CACAO 7573
BRADI:I1I1F5	enables	GO:0047763: caffeate O-methyltransferase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
ORYSJ:OMT1	enables	GO:0047763: caffeate O-methyltransferase activity	ECO:0000317: genomic context evidence used in manual assertion	UniProtKB:I1I1F5 UniProtKB:Q84X55	F		Seeded From UniProt	complete
ORYSJ:OMT1		GO:0047763: caffeate O-methyltransferase activity	ECO:0000317:	UniProtKB:I1I1F5 UniProtKB:Q84X55	F		As seen in figure 1, collinearity analysis revealed orthologous relationship among Brachypodium BdCOMT4, rice COMT and sorghum COMT.	complete CACAO 7721

References

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