PMID:23401858

Citation	Xue, YM, Kowalska, AK, Grabowska, K, Przybyt, K, Cichewicz, MA, Del Rosario, BC and Pemberton, LF (2013) Histone Chaperones Nap1 and Vps75 Regulate Histone Acetylation during Transcription Elongation. Mol. Cell. Biol. 33:1645-56
Abstract	Histone chaperones function in chromatin assembly and disassembly, suggesting they have important regulatory roles in transcription elongation. The Saccharomyces cerevisiae proteins Nap1 and Vps75 are structurally related, evolutionarily conserved histone chaperones. We showed that Nap1 genetically interacts with several transcription elongation factors and that both Nap1 and Vps75 interact with the RNA polymerase II kinase, CTK1. Loss of NAP1 or VPS75 suppressed cryptic transcription within the open reading frame (ORF) observed when strains are deleted for the kinase CTK1. Loss of the histone acetyltransferase Rtt109 also suppressed ctk1-dependent cryptic transcription. Vps75 regulates Rtt109 function, suggesting that they function together in this process. Histone H3 K9 was found to be the important lysine that is acetylated by Rtt109 during ctk1-dependent cryptic transcription. We showed that both Vps75 and Nap1 regulate the relative level of H3 K9 acetylation in the STE11 ORF. This supports a model in which Nap1, like Vps75, directly regulates Rtt109 activity or regulates the assembly of acetylated chromatin. Although Nap1 and Vps75 share many similarities, due to their distinct interactions with SET2, Nap1 and Vps75 may also play separate roles during transcription elongation. This work sheds further light on the importance of histone chaperones as general regulators of transcription elongation.
Links	PubMed Online version:10.1128/MCB.01121-12
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:RPB1		GO:0035067: negative regulation of histone acetylation	ECO:0000315:		P		Figure 7 shows ctk1 mutants had increased histone acetylation.	complete CACAO 7233
YEAST:VPS75		GO:2000617: positive regulation of histone H3-K9 acetylation	ECO:0000315:		P		Figures 4, 5, and 6 describe the role of Vps75 in cryptic transcription and should be considered when analyzing Figure 7. Figure 7 shows the regulation of histone acetylation.	complete CACAO 7231
YEAST:VPS75	involved_in	GO:2000617: positive regulation of histone H3-K9 acetylation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
YEAST:NAP1		GO:2000617: positive regulation of histone H3-K9 acetylation	ECO:0000315:		P		Figures 1 and 4 describe the role of Nap1 in transcription and should be considered when analyzing Figure 7. Figure 7 shows that Nap1 regulates histone acetylation.	complete CACAO 7232
YEAST:NAP1	involved_in	GO:2000617: positive regulation of histone H3-K9 acetylation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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