PMID:23304523

Citation	Kuzu, SB, Güvenmez, HK and Denizci, AA (2012) Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51. Biotechnol Res Int 2012:135498
Abstract	This paper reports the isolation and identification of chitinase-producing Bacillus from chitin-containing wastes, production of a thermostable and alkaline chitinasese, and enzyme characterization. Bacillus thuringiensis subsp. kurstaki HBK-51 was isolated from soil and was identified. Chitinase was obtained from supernatant of B. thuringiensis HBK-51 strain and showed its optimum activity at 110°C and at pH 9.0. Following 3 hours of incubation period, the enzyme showed a high level of activity at 110°C (96% remaining activity) and between pH 9.0 and 12.0 (98% remaining activity). Considering these characteristics, the enzyme was described as hyperthermophile-thermostable and highly alkaline. Two bands of the enzyme weighing 50 and 125 kDa were obtained following 12% SDS-PAGE analyses. Among the metal ions and chemicals used, Ni(2+) (32%), K(+) (44%), and Cu(2+) (56%) increased the enzyme activity while EDTA (7%), SDS (7%), Hg(2+) (11%), and ethyl-acetimidate (20%) decreased the activity of the enzyme. Bacillus thuringiensis subsp. kurstaki HBK-51 is an important strain which can be used in several biotechnological applications as a chitinase producer.
Links	PubMed PMC3532916 Online version:10.1155/2012/135498
Keywords
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACTK:D0EW64	enables	GO:0004568: chitinase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
BACTK:D0EW64		GO:0004568: chitinase activity	ECO:0000314:		F		Fig. 1	complete CACAO 8279

References

See Help:References for how to manage references in GONUTS.