GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:23178120

From GONUTS
Jump to: navigation, search
Citation

Osmundson, J, Montero-Diez, C, Westblade, LF, Hochschild, A and Darst, SA (2012) Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein. Cell 151:1005-16

Abstract

Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) σ(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-Å-resolution crystal structure of the complex between gp67 and Sau σ(A) domain 4 (σ(A)(4)) explains how gp67 joins the RNAP promoter complex through σ(A)(4) without significantly affecting σ(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, σ(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP α-subunit C-terminal domain (αCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (σ(A)), and selectively affects the function of another subunit (αCTD) depending on promoter usage.

Links

PubMed PMC3604623 Online version:10.1016/j.cell.2012.10.034

Keywords

Base Sequence; Crystallography, X-Ray; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/metabolism; Growth Inhibitors/metabolism; Models, Molecular; Molecular Sequence Data; Myoviridae/metabolism; Promoter Regions, Genetic; RNA, Bacterial/genetics; RNA, Ribosomal/genetics; Sigma Factor/metabolism; Staphylococcus aureus/genetics; Staphylococcus aureus/growth & development; Staphylococcus aureus/virology; Transcription, Genetic; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

9CAUD:Q4Z9Y5

GO:0039602: suppression by virus of host transcription initiation from RNA polymerase II promoter

ECO:0000314:

P

The authors tested the effect of staphylococcus phage G1 gp67 on transcription of different types of bacteria. In figure 1 of the paper linked, it is shown that the addition of the protein gp67 inhibits the transcription of RNA in Staphylococcus aureus, but has no effect on the other bacteria tested upon.

complete
CACAO 11627


See also

References

See Help:References for how to manage references in GONUTS.