PMID:23171345

Citation	Chauvin, A, Caldelari, D, Wolfender, JL and Farmer, EE (2013) Four 13-lipoxygenases contribute to rapid jasmonate synthesis in wounded Arabidopsis thaliana leaves: a role for lipoxygenase 6 in responses to long-distance wound signals. New Phytol. 197:566-75
Abstract	Damage-inducible defenses in plants are controlled in part by jasmonates, fatty acid-derived regulators that start to accumulate within 30 s of wounding a leaf. Using liquid chromatography-tandem mass spectrometry, we sought to identify the 13-lipoxygenases (13-LOXs) that initiate wound-induced jasmonate synthesis within a 190-s timeframe in Arabidopsis thaliana in 19 single, double, triple and quadruple mutant combinations derived from the four 13-LOX genes in this plant. All four 13-LOXs were found to contribute to jasmonate synthesis in wounded leaves: among them LOX6 showed a unique behavior. The relative contribution of LOX6 to jasmonate synthesis increased with distance from a leaf tip wound, and LOX6 was the only 13-LOX necessary for the initiation of early jasmonate synthesis in leaves distal to the wounded leaf. Herbivory assays that compared Spodoptera littoralis feeding on the lox2-1 lox3B lox4A lox6A quadruple mutant and the lox2-1 lox3B lox4A triple mutant revealed a role for LOX6 in defense of the shoot apical meristem. Consistent with this, we found that LOX6 promoter activity was strong in the apical region of rosettes. The LOX6 promoter was active in and near developing xylem cells and in expression domains we term subtrichomal mounds.
Links	PubMed Online version:10.1111/nph.12029
Keywords	Animals; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/immunology; Arabidopsis/parasitology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cyclopentanes/metabolism; Gene Expression Regulation, Plant; Herbivory/immunology; Isoleucine/analogs & derivatives; Isoleucine/metabolism; Kinetics; Lipoxygenase/genetics; Lipoxygenase/metabolism; Mutation/genetics; Nuclear Proteins/metabolism; Oxylipins/metabolism; Plant Leaves/enzymology; Plant Leaves/parasitology; Promoter Regions, Genetic/genetics; Signal Transduction; Spodoptera/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:LOX6		GO:0009695: jasmonic acid biosynthetic process	ECO:0000315:		P		Figure 1	complete CACAO 8408
ARATH:LOX6		GO:0009611: response to wounding	ECO:0000315:		P		Figure 1 shows that in wild-type leaf 8, a significant JA accumulation response occurs 40 seconds after wounding, and 90 seconds after in leaf 13. In the lox6A mutant, the JA accumulation response was delayed 50 seconds after wounding in leaf 8 and was also delayed in leaf 13.	complete CACAO 8409
ARATH:LOX6	involved_in	GO:0009611: response to wounding	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:LOX6	involved_in	GO:0009695: jasmonic acid biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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