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PMID:23077625
| Citation |
Katz, G, Wei, H, Alimova, A, Katz, A, Morgan, DG and Gottlieb, P (2012) Protein P7 of the cystovirus φ6 is located at the three-fold axis of the unexpanded procapsid. PLoS ONE 7:e47489 |
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| Abstract |
The objective of this study was to determine the location of protein P7, the RNA packaging factor, in the procapsid of the φ6 cystovirus. A comparison of cryo-electron microscopy high-resolution single particle reconstructions of the φ6 complete unexpanded procapsid, the protein P2-minus procapsid (P2 is the RNA directed RNA-polymerase), and the P7-minus procapsid, show that prior to RNA packaging the P7 protein is located near the three-fold axis of symmetry. Difference maps highlight the precise position of P7 and demonstrate that in P7-minus particles the P2 proteins are less localized with reduced densities at the three-fold axes. We propose that P7 performs the mechanical function of stabilizing P2 on the inner protein P1 shell which ensures that entering viral single-stranded RNA is replicated. |
| Links |
PubMed PMC3471842 Online version:10.1371/journal.pone.0047489 |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0046729: viral procapsid |
ECO:0000314: |
C |
P4 is attached to the P1 shell. Figures 4 C,D and 5 C,D. |
complete | ||||
|
part_of |
GO:0046729: viral procapsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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