PMID:23075784

Citation	Ding, H, Zeng, H, Huang, L, Dong, Y, Duan, Y, Mao, X, Guo, G and Zou, Q (2012) Helicobacter pylori chaperone-like protein CagT plays an essential role in the translocation of CagA into host cells. J. Microbiol. Biotechnol. 22:1343-9
Abstract	Most of the Helicobacter pylori strains containing the cag pathogenicity island (PAI) have been associated with more severe gastric disease in infected humans. The cag PAI is composed of 27 proteins, and some of the components are required for CagA translocation into host cells as well as induction of proinflammatory cytokines, such as interleukin-8 (IL-8); however, the exact function of most of the components remains unknown or poorly characterized. In this study, we demonstrated that CagT (HP0532), which is an essential structural component of the cag PAI apparatus, plays an important role in the translocation of CagA into host epithelial cells. In addition to being located on the bacterial surface, CagT is also partially localized in the inner membrane, where it acts as a chaperone-like protein and promotes CagA translocation. However, CagT secretion was not detected by immunoprecipitation analysis of cell culture supernatants. Meanwhile, CagT was related to the introduction of IL-8 of the host cell. These results suggest that CagT is expressed on both the inner and outer bacterial membranes, where it serves as a unique type IV secretion system component that is involved in CagA secretion and cag PAI apparatus assembly.
Links	PubMed
Keywords	Antigens, Bacterial/genetics; Antigens, Bacterial/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Secretion Systems; Bacterial Translocation; Cell Line, Tumor; Cell Membrane/metabolism; Epithelium/microbiology; Genomic Islands; Helicobacter Infections/metabolism; Helicobacter Infections/microbiology; Helicobacter pylori/genetics; Helicobacter pylori/metabolism; Helicobacter pylori/pathogenicity; Helicobacter pylori/physiology; Humans; Immunoprecipitation; Interleukin-8/metabolism; Molecular Chaperones/metabolism; Phosphorylation; Plasmids/genetics; Plasmids/metabolism; Protein Interaction Mapping; Protein Transport; Virulence Factors/genetics; Virulence Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HELPY:CAGT	involved_in	GO:0009306: protein secretion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HELPY:CAGT	part_of	GO:0019867: outer membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
HELPY:CAGT		GO:0009306: protein secretion	ECO:0000315:		P		Figure 1 shows mutant variants in CagT which significantly have lowered translocation of CagA	complete CACAO 10104
HELPY:CAGT		GO:0070258: beta-tubulin folding	ECO:0000314:		P		Figure 2 shows cellular localization of CagT	complete CACAO 10105
HELPY:CAGT		GO:0019867: outer membrane	ECO:0000314:		C		Figure 2 shows cellular localization of CagT	complete CACAO 10106
HELPY:CAGT		GO:0005515: protein binding	ECO:0000314:		F		Figure 3 shows interaction with CagA	complete CACAO 10107

References

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