GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:23073013
| Citation |
Shibasaka, M, Sasano, S, Utsugi, S and Katsuhara, M (2012) Functional characterization of a novel plasma membrane intrinsic protein2 in barley. Plant Signal Behav 7:1648-52 |
|---|---|
| Abstract |
Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future. |
| Links |
PubMed PMC3578906 Online version:10.4161/psb.22294 |
| Keywords |
Animals; Cell Membrane/genetics; Cell Membrane/metabolism; Gene Expression Regulation, Plant/genetics; Gene Expression Regulation, Plant/physiology; Hordeum/genetics; Hordeum/metabolism; Onions/genetics; Onions/metabolism; Oocytes/metabolism; Oryza sativa/metabolism; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Water/metabolism; Xenopus laevis/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005886: membrane |
ECO:0000314: |
C |
Figure 4 shows EGFP-tagged HvPIP2.8 localizing to the membrane |
complete | ||||
| GO:0005372: water transmembrane transporter activity |
ECO:0000315: |
F |
Figure 2 shows that oocytes expressing HvPIP2;8 have an increased water permeability coefficient than those expressing an empty control. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
